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SNF8 Monoclonal antibody

SNF8 Monoclonal Antibody for IF, IHC, WB,ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human and More (1)

Applications

WB, IHC, IF, ELISA

Conjugate

Unconjugated

CloneNo.

2C10F2

Cat no : 67696-1-Ig

Synonyms

Dot3, EAP30, ESCRT II complex subunit VPS22, hVps22, SNF8, Vacuolar sorting protein SNF8, VPS22

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using SNF8 Monoclonal antibody (67696-1-Ig)

    WB analysis using 67696-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 67696-1-Ig (SNF8 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human breast cancer tissue using SNF8 Monoclonal antibody (67696-1-Ig)

    IHC staining of human breast cancer using 67696-1-Ig

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 67696-1-Ig (SNF8 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human breast cancer tissue using SNF8 Monoclonal antibody (67696-1-Ig)

    IHC staining of human breast cancer using 67696-1-Ig

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 67696-1-Ig (SNF8 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using SNF8 Monoclonal antibody (67696-1-Ig)

    IF Staining of HeLa using 67696-1-Ig

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using SNF8 antibody (67696-1-Ig, Clone: 2C10F2 ) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L), CL594-Phalloidin (red).

Tested Applications

Positive WB detected inHeLa cells, K-562 cells
Positive IHC detected inhuman breast cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:3000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)IF : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

IFSee 1 publications below

Product Information

67696-1-Ig targets SNF8 in WB, IHC, IF, ELISA applications and shows reactivity with Human samples.

Tested Reactivity Human
Cited Reactivityhuman
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen SNF8 fusion protein Ag30384
Full Name SNF8, ESCRT-II complex subunit, homolog (S. cerevisiae)
Calculated Molecular Weight 258 aa, 29 kDa
Observed Molecular Weight29 kDa
GenBank Accession NumberBC008976
Gene Symbol SNF8
Gene ID (NCBI) 11267
RRIDAB_2882888
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Protocols

Product Specific Protocols
WB protocol for SNF8 antibody 67696-1-IgDownload protocol
IHC protocol for SNF8 antibody 67696-1-IgDownload protocol
IF protocol for SNF8 antibody 67696-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanIF

Nat Commun

Rab41-mediated ESCRT machinery repairs membrane rupture by a bacterial toxin in xenophagy

Authors - Takashi Nozawa
View Article