• All
  • Primary Antibodies
  • Conjugated Antibodies for IF
  • Conjugated Antibodies for FC
  • Secondary Antibodies
  • Antibody Labeling KitsNew
  • ELISA Kits
  • IHC Kits
  • Magnetic Cell Separation Kits
  • Cytokines & Growth Factors
  • Neutralizing/activating Antibodies
  • Nanobody-based Reagents
  • Accessory Products and Kits
  • Fusion Proteins
×
×
Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0

SPAG4-Specific Polyclonal antibody

SPAG4-Specific Polyclonal Antibody for IF, IHC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, IF, ELISA

Conjugate

Unconjugated

Cat no : 19721-1-AP

Synonyms

SPAG4, SPAG4-Specific, sperm associated antigen 4, SUN4

Validation Data Gallery

Filter:
  • Western Blot (WB) analysis of human testis tissue using SPAG4-Specific Polyclonal antibody (19721-1-AP)

    WB analysis of human testis using 19721-1-AP

    human testis tissue were subjected to SDS PAGE followed by western blot with 19721-1-AP (SPAG4-Specific Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HepG2 cells using SPAG4-Specific Polyclonal antibody (19721-1-AP)

    WB analysis of HepG2 using 19721-1-AP

    HepG2 cells were subjected to SDS PAGE followed by western blot with 19721-1-AP (SPAG4-Specific Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of human testis tissue using SPAG4-Specific Polyclonal antibody (19721-1-AP)

    WB analysis of human testis using 19721-1-AP

    human testis tissue were subjected to SDS PAGE followed by western blot with 19721-1-AP (SPAG4-Specific Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of mouse testis tissue using SPAG4-Specific Polyclonal antibody (19721-1-AP)

    IHC staining of mouse testis using 19721-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 19721-1-AP (SPAG4-Specific antibody) at dilution of 1:100 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of mouse testis tissue using SPAG4-Specific Polyclonal antibody (19721-1-AP)

    IHC staining of mouse testis using 19721-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 19721-1-AP (SPAG4-Specific antibody) at dilution of 1:100 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunofluorescence (IF) / fluorescent staining of mouse testis tissue using SPAG4-Specific Polyclonal antibody (19721-1-AP)

    IF Staining of mouse testis using 19721-1-AP

    Immunofluorescent analysis of (4% PFA) fixed mouse testis tissue using SPAG4-Specific antibody (19721-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using SPAG4-Specific Polyclonal antibody (19721-1-AP)

    IF Staining of HepG2 using 19721-1-AP

    Immunofluorescent analysis of HepG2 cells using 19721-1-AP (SPAG4-Specific antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

Tested Applications

Positive WB detected inhuman testis tissue, HepG2 cells
Positive IHC detected inmouse testis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inmouse testis tissue, HepG2 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)IF : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

WBSee 2 publications below

Product Information

19721-1-AP targets SPAG4-Specific in WB, IHC, IF, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Cited Reactivityhuman, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Peptide
Full Name sperm associated antigen 4
Calculated Molecular Weight 48 kDa
Observed Molecular Weight 55 kDa
GenBank Accession NumberNM_003116
Gene Symbol SPAG4
Gene ID (NCBI) 6676
RRIDAB_2878600
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

SPAG4 may assist the organization and assembly of outer dense fibers (ODFs), a specific structure of the sperm tail. This antibody is specific to SPAG4. We observed that the molecular weight of SPAG4-Specific was 55 kDa.

Protocols

Product Specific Protocols
WB protocol for SPAG4-Specific antibody 19721-1-APDownload protocol
IHC protocol for SPAG4-Specific antibody 19721-1-APDownload protocol
IF protocol for SPAG4-Specific antibody 19721-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouseWB

J Biol Chem

The testis-specific LINC component SUN3 is essential for sperm head shaping during mouse spermiogenesis.

Authors - Qian Gao
View Article
humanWB

Cancer Cell Int

Novel risk model based on angiogenesis-related lncRNAs for prognosis prediction of hepatocellular carcinoma

Authors - Shicheng Xie
View Article