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- KD/KO Validated
SPAG4L Polyclonal antibody
SPAG4L Polyclonal Antibody for WB, IF, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse
Applications
WB, IF, ELISA
Conjugate
Unconjugated
Cat no : 17495-1-AP
Synonyms
Validation Data Gallery
Tested Applications
| Positive WB detected in | PC-3 cells |
| Positive IF detected in | PC-3 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:1000 |
| Immunofluorescence (IF) | IF : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| KD/KO | See 1 publications below |
| WB | See 8 publications below |
| IF | See 11 publications below |
Product Information
17495-1-AP targets SPAG4L in WB, IF, ELISA applications and shows reactivity with human, mouse samples.
| Tested Reactivity | human, mouse |
| Cited Reactivity | human, mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | SPAG4L fusion protein Ag11629 |
| Full Name | sperm associated antigen 4-like |
| Calculated Molecular Weight | 379 aa, 43 kDa |
| Observed Molecular Weight | 43 kDa |
| GenBank Accession Number | BC026118 |
| Gene Symbol | SPAG4L |
| Gene ID (NCBI) | 140732 |
| RRID | AB_1939754 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
SPAG4L,also named as SUN domain-containing protein 5 or Testis and spermatogenesis-related gene 4 protein, is a 379 amino acid protein, which contains 1 SUN domain. SPAG4L is widely expressed and localizes in the nucleus inner membrane. SPAG4L is restricted to the apical nuclear region of round spermatids that face the acrosomic vesicle. SPAG4L is involved in linkage of the acrosomic vesicle to the spermatid nucleus, and in acrosome biogenesis.
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for SPAG4L antibody 17495-1-AP | Download protocol |
| IF protocol for SPAG4L antibody 17495-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Nat Commun The missing linker between SUN5 and PMFBP1 in sperm head-tail coupling apparatus. | ||
Am J Hum Genet Biallelic SUN5 Mutations Cause Autosomal-Recessive Acephalic Spermatozoa Syndrome. | ||
Int J Mol Sci Identification and Characterization of the Most Common Genetic Variant Responsible for Acephalic Spermatozoa Syndrome in Men Originating from North Africa. | ||
Cancers (Basel) Nuclear Membrane Protein SUN5 Is Highly Expressed and Promotes Proliferation and Migration in Colorectal Cancer by Regulating the ERK Pathway |
