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SPAG7 Polyclonal antibody

SPAG7 Polyclonal Antibody for IHC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 16294-1-AP

Synonyms

ACRP, FSA 1, SPAG7, sperm associated antigen 7

Validation Data Gallery

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  • Western Blot (WB) analysis of mouse ovary tissue using SPAG7 Polyclonal antibody (16294-1-AP)

    WB analysis of mouse ovary using 16294-1-AP

    mouse ovary tissue were subjected to SDS PAGE followed by western blot with 16294-1-AP (SPAG7 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of various lysates using SPAG7 Polyclonal antibody (16294-1-AP)

    WB analysis using 16294-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 16294-1-AP (SPAG7 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of mouse brain tissue using SPAG7 Polyclonal antibody (16294-1-AP)

    WB analysis of mouse brain using 16294-1-AP

    mouse brain tissue were subjected to SDS PAGE followed by western blot with 16294-1-AP (SPAG7 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of mouse skeletal muscle tissue using SPAG7 Polyclonal antibody (16294-1-AP)

    WB analysis of mouse skeletal muscle using 16294-1-AP

    mouse skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 16294-1-AP (SPAG7 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human colon cancer tissue using SPAG7 Polyclonal antibody (16294-1-AP)

    IHC staining of human colon cancer using 16294-1-AP

    Immunohistochemical analysis of paraffin-embedded human colon cancer using 16294-1-AP (SPAG7 antibody) at dilution of 1:50 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human colon cancer tissue using SPAG7 Polyclonal antibody (16294-1-AP)

    IHC staining of human colon cancer using 16294-1-AP

    Immunohistochemical analysis of paraffin-embedded human colon cancer using 16294-1-AP (SPAG7 antibody) at dilution of 1:50 (under 40x lens).

Tested Applications

Positive WB detected inmouse ovary tissue, mouse brain tissue, mouse skeletal muscle tissue, rat brain tissue
Positive IHC detected inhuman colon cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

16294-1-AP targets SPAG7 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen SPAG7 fusion protein Ag9471
Full Name sperm associated antigen 7
Calculated Molecular Weight 227 aa, 26 kDa
Observed Molecular Weight 26-31 kDa
GenBank Accession NumberBC011934
Gene Symbol SPAG7
Gene ID (NCBI) 9552
RRIDAB_2302478
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

SPAG7 encodes the sperm-associated antigen 7, which is widely expressed. The SPAG7 protein contains two nuclear localisation signals and a R3H domain. The domain is predicted to bind ssDNA or ssRNA in a sequence-specific manner. This might indicate a potential role of the protein in response to viruses or free DNAs or RNAs.

Protocols

Product Specific Protocols
WB protocol for SPAG7 antibody 16294-1-APDownload protocol
IHC protocol for SPAG7 antibody 16294-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols