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SPANXA2/SPANXA1 Polyclonal antibody

SPANXA2/SPANXA1 Polyclonal Antibody for IF, IHC, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, IF,ELISA

Conjugate

Unconjugated

Cat no : 19527-1-AP

Synonyms

Cancer/testis antigen 11.1, CT11.1, SPAN X, SPANX, SPANX A, SPANX family member A, SPANX family, member A2, SPANXA, SPANXA1, SPANXA2

Validation Data Gallery

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  • Western Blot (WB) analysis of DU 145 cells using SPANXA2/SPANXA1 Polyclonal antibody (19527-1-AP)

    WB analysis of DU 145 using 19527-1-AP

    DU 145 cells were subjected to SDS PAGE followed by western blot with 19527-1-AP (SPANXA2/SPANXA1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of PC-3 cells using SPANXA2/SPANXA1 Polyclonal antibody (19527-1-AP)

    WB analysis of PC-3 using 19527-1-AP

    PC-3 cells were subjected to SDS PAGE followed by western blot with 19527-1-AP (SPANXA2/SPANXA1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of PC-3 cells using SPANXA2/SPANXA1 Polyclonal antibody (19527-1-AP)

    WB analysis of PC-3 using 19527-1-AP

    PC-3 cells were subjected to SDS PAGE followed by western blot with 19527-1-AP (SPANXA2/SPANXA1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HEK-293 cells using SPANXA2/SPANXA1 Polyclonal antibody (19527-1-AP)

    WB analysis of HEK-293 using 19527-1-AP

    HEK-293 cells were subjected to SDS PAGE followed by western blot with 19527-1-AP (SPANXA2/SPANXA1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human testis tissue using SPANXA2/SPANXA1 Polyclonal antibody (19527-1-AP)

    IHC staining of human testis using 19527-1-AP

    Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 19527-1-AP (SPANXA2/SPANXA1 antibody at dilution of 1:50.

  • Immunohistochemistry (IHC) staining of human skin cancer tissue using SPANXA2/SPANXA1 Polyclonal antibody (19527-1-AP)

    IHC staining of human skin cancer using 19527-1-AP

    Immunohistochemical analysis of paraffin-embedded human skin cancer tissue slide using 19527-1-AP (SPANXA2/SPANXA1 antibody at dilution of 1:50.

  • Immunohistochemistry (IHC) staining of human skin cancer tissue using SPANXA2/SPANXA1 Polyclonal antibody (19527-1-AP)

    IHC staining of human skin cancer using 19527-1-AP

    Immunohistochemical analysis of paraffin-embedded human skin cancer tissue slide using 19527-1-AP (SPANXA2/SPANXA1 antibody at dilution of 1:50.

  • Immunohistochemistry (IHC) staining of human testis tissue using SPANXA2/SPANXA1 Polyclonal antibody (19527-1-AP)

    IHC staining of human testis using 19527-1-AP

    Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 19527-1-AP (SPANXA2/SPANXA1 antibody at dilution of 1:50.

  • Immunofluorescence (IF) / fluorescent staining of HEK-293 cells using SPANXA2/SPANXA1 Polyclonal antibody (19527-1-AP)

    IF Staining of HEK-293 using 19527-1-AP

    Immunofluorescent analysis of HEK-293 cells using 19527-1-AP (SPANXA2/SPANXA1 antibody) at dilution of 1:25 and Rhodamine-Goat anti-Rabbit IgG.

Tested Applications

Positive WB detected inDU 145 cells, HEK-293 cells, PC-3 cells
Positive IHC detected inhuman skin cancer tissue, human testis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHEK-293 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)IF : 1:10-1:100
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

19527-1-AP targets SPANXA2/SPANXA1 in WB, IHC, IF,ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Peptide
Full Name SPANX family, member A2
Calculated Molecular Weight 11 kDa
Observed Molecular Weight 27 kDa
GenBank Accession NumberNM_145662
Gene Symbol SPANXA2
Gene ID (NCBI) 728712
RRIDAB_2878586
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

SPANXA1, also named as SPANXA and SPANXA2, is a member of the SPANX family of cancer/testis-associated genes. It encodes differentially expressed testis-specific proteins that localize to various subcellular compartments. On Western blots of human testis proteins, SPANX antibodies reacts with the bands migrating between 15-30 kDa. The SPANX protein may be a useful target in cancer immunotherapy.

Protocols

Product Specific Protocols
WB protocol for SPANXA2/SPANXA1 antibody 19527-1-APDownload protocol
IHC protocol for SPANXA2/SPANXA1 antibody 19527-1-APDownload protocol
IF protocol for SPANXA2/SPANXA1 antibody 19527-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols