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ST13 Polyclonal antibody

ST13 Polyclonal Antibody for IHC, IP, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IP, IHC, ELISA

Conjugate

Unconjugated

Cat no : 26581-1-AP

Synonyms

AAG2, FAM10A1, FAM10A4, HIP, HOP, Hsc70 interacting protein, HSPABP, HSPABP1, P48, PRO0786, Protein FAM10A1, Putative tumor suppressor ST13, SNC6, ST13

Validation Data Gallery

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  • Western Blot (WB) analysis of HEK-293 cells using ST13 Polyclonal antibody (26581-1-AP)

    WB analysis of HEK-293 using 26581-1-AP

    HEK-293 cells were subjected to SDS PAGE followed by western blot with 26581-1-AP (ST13 Antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of mouse testis tissue using ST13 Polyclonal antibody (26581-1-AP)

    WB analysis of mouse testis using 26581-1-AP

    mouse testis tissue were subjected to SDS PAGE followed by western blot with 26581-1-AP (ST13 Antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • Immunoprecipitation (IP) experiment of HEK-293 cells using ST13 Polyclonal antibody (26581-1-AP)

    IP experiment of HEK-293 using 26581-1-AP

    IP result of anti-ST13 (IP:26581-1-AP, 4ug; Detection:26581-1-AP 1:2000) with HEK-293 cells lysate 1800ug.

  • Immunohistochemistry (IHC) staining of human colon cancer tissue using ST13 Polyclonal antibody (26581-1-AP)

    IHC staining of human colon cancer using 26581-1-AP

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 26581-1-AP (ST13 Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human colon cancer tissue using ST13 Polyclonal antibody (26581-1-AP)

    IHC staining of human colon cancer using 26581-1-AP

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 26581-1-AP (ST13 Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

Tested Applications

Positive WB detected inHEK-293 cells, mouse testis tissue
Positive IP detected inHEK-293 cells
Positive IHC detected inhuman colon cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:6000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

The immunogen of 26581-1-AP is ST13 Fusion Protein expressed in E. coli.

Tested Reactivity human, mouse
Cited Reactivityhuman, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen ST13 fusion protein Ag24779
Full Name suppression of tumorigenicity 13 (colon carcinoma) (Hsp70 interacting protein)
Observed Molecular Weight 45-50 kDa
GenBank Accession NumberBC139724
Gene Symbol ST13
Gene ID (NCBI) 6767
RRIDAB_2880560
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Protocols

Product Specific Protocols
WB protocol for ST13 antibody 26581-1-APDownload protocol
IHC protocol for ST13 antibody 26581-1-APDownload protocol
IP protocol for ST13 antibody 26581-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanIHC

Sci Rep

Novel candidate factors predicting the effect of S-1 adjuvant chemotherapy of pancreatic cancer.

Authors - Katsutaka Mitachi
View Article
mouseWB

Cancer Res

N-myc-Mediated Translation Control Is a Therapeutic Vulnerability in Medulloblastoma

Authors - Duygu Kuzuoglu-Ozturk
View Article