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STARD5 Polyclonal antibody

STARD5 Polyclonal Antibody for IF, IHC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, IF, ELISA

Conjugate

Unconjugated

Cat no : 10487-1-AP

Synonyms

STARD5

Validation Data Gallery

Filter:
  • Western Blot (WB) analysis of mouse liver tissue using STARD5 Polyclonal antibody (10487-1-AP)

    WB analysis of mouse liver using 10487-1-AP

    mouse liver tissue were subjected to SDS PAGE followed by western blot with 10487-1-AP (STARD5 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of various lysates using STARD5 Polyclonal antibody (10487-1-AP)

    WB analysis using 10487-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 10487-1-AP (STARD5 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human liver tissue using STARD5 Polyclonal antibody (10487-1-AP)

    IHC staining of human liver using 10487-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver using 10487-1-AP (STARD5 antibody) at dilution of 1:50 (under 40x lens).

  • Immunohistochemistry (IHC) staining of human liver tissue using STARD5 Polyclonal antibody (10487-1-AP)

    IHC staining of human liver using 10487-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver using 10487-1-AP (STARD5 antibody) at dilution of 1:50 (under 10x lens).

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using STARD5 Polyclonal antibody (10487-1-AP)

    IF Staining of HepG2 using 10487-1-AP

    Immunofluorescent analysis of HepG2 cells using 10487-1-AP (STARD5 antibody) at dilution of 1:25 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

Tested Applications

Positive WB detected inCOLO 320 cells, mouse liver tissue, HEK-293T cells, HepG2 cells, THP-1 cells
Positive IHC detected inhuman liver tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHepG2 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)IF : 1:10-1:100
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

WBSee 1 publications below

Product Information

10487-1-AP targets STARD5 in WB, IHC, IF, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Cited Reactivitymouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen STARD5 fusion protein Ag0753
Full Name StAR-related lipid transfer (START) domain containing 5
Calculated Molecular Weight 23.4 kDa
Observed Molecular Weight 24-26 kDa
GenBank Accession NumberBC004365
Gene Symbol STARD5
Gene ID (NCBI) 80765
RRIDAB_2197808
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Protocols

Product Specific Protocols
WB protocol for STARD5 antibody 10487-1-APDownload protocol
IHC protocol for STARD5 antibody 10487-1-APDownload protocol
IF protocol for STARD5 antibody 10487-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouseWB

PLoS One

Relationships between hematopoiesis and hepatogenesis in the midtrimester fetal liver characterized by dynamic transcriptomic and proteomic profiles.

Authors - Yuanbiao Guo
View Article