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Syntaxin 8 Polyclonal antibody

Syntaxin 8 Polyclonal Antibody for FC, IF, IP, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, IF, FC, CoIP, ELISA

Conjugate

Unconjugated

Cat no : 12206-1-AP

Synonyms

CARB, STX8, syntaxin 8

Validation Data Gallery

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  • Western Blot (WB) analysis of human heart tissue using Syntaxin 8 Polyclonal antibody (12206-1-AP)

    WB analysis of human heart using 12206-1-AP

    human heart tissue were subjected to SDS PAGE followed by western blot with 12206-1-AP (Syntaxin 8 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of human kidney tissue using Syntaxin 8 Polyclonal antibody (12206-1-AP)

    WB analysis of human kidney using 12206-1-AP

    human kidney tissue were subjected to SDS PAGE followed by western blot with 12206-1-AP (Syntaxin 8 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of mouse pancreas tissue using Syntaxin 8 Polyclonal antibody (12206-1-AP)

    WB analysis of mouse pancreas using 12206-1-AP

    mouse pancreas tissue were subjected to SDS PAGE followed by western blot with 12206-1-AP (Syntaxin 8 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of human lung tissue using Syntaxin 8 Polyclonal antibody (12206-1-AP)

    WB analysis of human lung using 12206-1-AP

    human lung tissue were subjected to SDS PAGE followed by western blot with 12206-1-AP (Syntaxin 8 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of A375 cells using Syntaxin 8 Polyclonal antibody (12206-1-AP)

    WB analysis of A375 using 12206-1-AP

    A375 cells were subjected to SDS PAGE followed by western blot with 12206-1-AP (Syntaxin 8 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of human brain tissue using Syntaxin 8 Polyclonal antibody (12206-1-AP)

    WB analysis of human brain using 12206-1-AP

    human brain tissue were subjected to SDS PAGE followed by western blot with 12206-1-AP (Syntaxin 8 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of human placenta tissue using Syntaxin 8 Polyclonal antibody (12206-1-AP)

    WB analysis of human placenta using 12206-1-AP

    human placenta tissue were subjected to SDS PAGE followed by western blot with 12206-1-AP (Syntaxin 8 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of human liver tissue using Syntaxin 8 Polyclonal antibody (12206-1-AP)

    WB analysis of human liver using 12206-1-AP

    human liver tissue were subjected to SDS PAGE followed by western blot with 12206-1-AP (Syntaxin 8 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Immunoprecipitation (IP) experiment of A375 cells using Syntaxin 8 Polyclonal antibody (12206-1-AP)

    IP experiment of A375 using 12206-1-AP

    IP result of anti-Syntaxin 8 (IP:12206-1-AP, 4ug; Detection:12206-1-AP 1:500) with A375 cells lysate 3600ug.

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using Syntaxin 8 Polyclonal antibody (12206-1-AP)

    IF Staining of HepG2 using 12206-1-AP

    Immunofluorescent analysis of HepG2 cells, using STX8 antibody 12206-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).

  • Flow cytometry (FC) experiment of HepG2 cells using Syntaxin 8 Polyclonal antibody (12206-1-AP)

    FC experiment of HepG2 using 12206-1-AP

    1X10^6 HepG2 cells were stained with 0.2ug Syntaxin 8 antibody (12206-1-AP, red) and control antibody (blue). Fixed with 90% MeOH blocked with 3% BSA (30 min). Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) with dilution 1:1000.

Tested Applications

Positive WB detected inhuman heart tissue, A375 cells, human brain tissue, human kidney tissue, human liver tissue, human lung tissue, human placenta tissue, mouse pancreas tissue
Positive IP detected inA375 cells
Positive IF detected inHepG2 cells
Positive FC detected inHepG2 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunofluorescence (IF)IF : 1:10-1:100
Flow Cytometry (FC)FC : 0.20 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

12206-1-AP targets Syntaxin 8 in WB, IP, IF, FC, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivitymouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Syntaxin 8 fusion protein Ag2850
Full Name syntaxin 8
Calculated Molecular Weight 236 aa, 27 kDa
Observed Molecular Weight 27 kDa
GenBank Accession NumberBC009713
Gene Symbol STX8
Gene ID (NCBI) 9482
RRIDAB_2196671
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Syntaxin 8 (STX8), also known as CIP-1-associated regulator of cyclin B (CARB ), is a protein belongs to the syntaxin family. The gene of STX8 maps to chromosomal band 17p12 and it encodes a 236-amino-acid protein containing 1 t-SNARE coiled-coil homology domain. STX8 mRNA is broadly expressed in multiple human tissues, including heart, brain, kidney, liver, lung, placenta, skeletal muscle, spleen and pancreas (PMID: 10198254). STX8 is involved in protein trafficking from early to late endosomes via vesicle fusion and exocytosis.

Protocols

Product Specific Protocols
WB protocol for Syntaxin 8 antibody 12206-1-APDownload protocol
IF protocol for Syntaxin 8 antibody 12206-1-APDownload protocol
IP protocol for Syntaxin 8 antibody 12206-1-APDownload protocol
FC protocol for Syntaxin 8 antibody 12206-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
ratWB,CoIP

J Cell Biol

VAMP4 regulates insulin levels by targeting secretory granules to lysosomes

Authors - Min Li
  • KD Validated
View Article
mouse

PLoS One

The Gag protein PEG10 binds to RNA and regulates trophoblast stem cell lineage specification.

Authors - Mona Abed
View Article