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STYK1 Polyclonal antibody

STYK1 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 18028-1-AP

Synonyms

NOK, Protein PK unique, STYK1, SuRTK106, Tyrosine protein kinase STYK1

Validation Data Gallery

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  • Western Blot (WB) analysis of human brain tissue using STYK1 Polyclonal antibody (18028-1-AP)

    WB analysis of human brain using 18028-1-AP

    human brain tissue were subjected to SDS PAGE followed by western blot with 18028-1-AP (STYK1 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HeLa cells using STYK1 Polyclonal antibody (18028-1-AP)

    WB analysis of HeLa using 18028-1-AP

    HeLa cells were subjected to SDS PAGE followed by western blot with 18028-1-AP (STYK1 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of mouse brain tissue using STYK1 Polyclonal antibody (18028-1-AP)

    IHC staining of mouse brain using 18028-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 18028-1-AP (STYK1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of mouse brain tissue using STYK1 Polyclonal antibody (18028-1-AP)

    IHC staining of mouse brain using 18028-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 18028-1-AP (STYK1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

Tested Applications

Positive WB detected inhuman brain tissue, HeLa cells
Positive IHC detected inmouse brain tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:3000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

WBSee 3 publications below

Product Information

18028-1-AP targets STYK1 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen STYK1 fusion protein Ag12594
Full Name serine/threonine/tyrosine kinase 1
Calculated Molecular Weight 422 aa, 48 kDa
Observed Molecular Weight 48 kDa
GenBank Accession NumberBC093822
Gene Symbol STYK1
Gene ID (NCBI) 55359
RRIDAB_10603477
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Protocols

Product Specific Protocols
WB protocol for STYK1 antibody 18028-1-APDownload protocol
IHC protocol for STYK1 antibody 18028-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouseWB

Oncol Lett

Downregulation of GLUT3 impairs STYK1/NOK-mediated metabolic reprogramming and proliferation in NIH-3T3 cells.

Authors - Weiye Shi
View Article
humanWB

Heliyon

STYK1/NOK affects cell cycle late mitosis and directly interacts with anaphase-promoting complex activator CDH1

Authors - Su-Lin Zeng
View Article
mouseWB

Biochem Biophys Res Commun

Induction of chronic lymphocytic leukemia-like disease in STYK1/NOK transgenic mice

Authors - Yin Yang
View Article