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Synaptojanin 2 Polyclonal antibody

Synaptojanin 2 Polyclonal Antibody for IF, IHC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF, ELISA

Conjugate

Unconjugated

Cat no : 13893-1-AP

Synonyms

INPP5H, KIAA0348, Synaptojanin 2, SYNJ2

Validation Data Gallery

Filter:
  • Western Blot (WB) analysis of various lysates using Synaptojanin 2 Polyclonal antibody (13893-1-AP)

    WB analysis using 13893-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 13893-1-AP (Synaptojanin 2 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of mouse small intestine tissue using Synaptojanin 2 Polyclonal antibody (13893-1-AP)

    IHC staining of mouse small intestine using 13893-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse small intestine tissue slide using 13893-1-AP (Synaptojanin 2 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human brain tissue using Synaptojanin 2 Polyclonal antibody (13893-1-AP)

    IHC staining of human brain using 13893-1-AP

    Immunohistochemical analysis of paraffin-embedded human brain tissue slide using 13893-1-AP (Synaptojanin 2 antibody at dilution of 1:50 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human brain tissue using Synaptojanin 2 Polyclonal antibody (13893-1-AP)

    IHC staining of human brain using 13893-1-AP

    Immunohistochemical analysis of paraffin-embedded human brain tissue slide using 13893-1-AP (Synaptojanin 2 antibody at dilution of 1:50 (under 40x lens).

  • Immunofluorescence (IF) / fluorescent staining of SH-SY5Y cells using Synaptojanin 2 Polyclonal antibody (13893-1-AP)

    IF Staining of SH-SY5Y using 13893-1-AP

    Immunofluorescent analysis of (-20℃ Ethanol) fixed SH-SY5Y cells using 13893-1-AP (Synaptojanin 2 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

Tested Applications

Positive WB detected inHEK-293 cells, Jurkat cells
Positive IHC detected inhuman brain tissue, mouse small intestine tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inSH-SY5Y cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)IF : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

WBSee 1 publications below

Product Information

13893-1-AP targets Synaptojanin 2 in WB, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivitymouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Synaptojanin 2 fusion protein Ag4855
Full Name synaptojanin 2
Calculated Molecular Weight 1496 aa, 166 kDa
Observed Molecular Weight143, 160 kDa
GenBank Accession NumberBC043277
Gene Symbol SYNJ2
Gene ID (NCBI) 8871
RRIDAB_2255784
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

SYNJ2(Synaptojanin-2) is also named as KIAA0348 and belongs to the synaptojanin family. SYNJ2 encodes an inositol polyphosphate phosphatase that functions in recycling neurotransmitter vesicles and is implicated in spermatogenesis(PMID: 24103750). This protein has 3 isoforms produced by alternative splicing.

Protocols

Product Specific Protocols
WB protocol for Synaptojanin 2 antibody 13893-1-APDownload protocol
IHC protocol for Synaptojanin 2 antibody 13893-1-APDownload protocol
IF protocol for Synaptojanin 2 antibody 13893-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouseWB

Neuron

Neuronal mitochondria transport Pink1 mRNA via synaptojanin 2 to support local mitophagy.

Authors - Angelika B Harbauer
View Article
mouse

Front Cardiovasc Med

Macrophages-Related Genes Biomarkers in the Deterioration of Atherosclerosis.

Authors - Yue Zheng
View Article