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SYNPO2L Polyclonal antibody

SYNPO2L Polyclonal Antibody for WB, IF, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IF, IHC, ELISA

Conjugate

Unconjugated

Cat no : 21480-1-AP

Synonyms

synaptopodin 2 like, Synaptopodin 2 like protein, SYNPO2L

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using SYNPO2L Polyclonal antibody (21480-1-AP)

    WB analysis using 21480-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 21480-1-AP (SYNPO2L antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of PC-3 cells using SYNPO2L Polyclonal antibody (21480-1-AP)

    WB analysis of PC-3 using 21480-1-AP

    PC-3 cells were subjected to SDS PAGE followed by western blot with 21480-1-AP (SYNPO2L antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of mouse testis tissue using SYNPO2L Polyclonal antibody (21480-1-AP)

    WB analysis of mouse testis using 21480-1-AP

    mouse testis tissue were subjected to SDS PAGE followed by western blot with 21480-1-AP (SYNPO2L antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of mouse heart tissue using SYNPO2L Polyclonal antibody (21480-1-AP)

    IHC staining of mouse heart using 21480-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 21480-1-AP (SYNPO2L antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of mouse heart tissue using SYNPO2L Polyclonal antibody (21480-1-AP)

    IHC staining of mouse heart using 21480-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 21480-1-AP (SYNPO2L antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using SYNPO2L Polyclonal antibody (21480-1-AP)

    IF Staining of HepG2 using 21480-1-AP

    Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using SYNPO2L antibody (21480-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

Tested Applications

Positive WB detected inmouse testis tissue, PC-3 cells, rat testis tissue
Positive IHC detected inmouse heart tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHepG2 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:3000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)IF : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

21480-1-AP targets SYNPO2L in WB, IF, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen SYNPO2L fusion protein Ag15683
Full Name synaptopodin 2-like
Calculated Molecular Weight 977 aa, 102 kDa
Observed Molecular Weight 79 kDa
GenBank Accession NumberBC142635
Gene Symbol SYNPO2L
Gene ID (NCBI) 79933
RRIDAB_10860260
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

SYNPO2L belongs to the synaptopodin family and is an actin-associated protein that may play a role in modulating actin-based shape. SYNPO2L is involved in the positive regulation of Rho protein signal transduction and the positive regulation of stress fiber assembly. Loss-of-function variants in the SYNPO2L gene are associated with atrial fibrillation (PMID: 33768119).

Protocols

Product Specific Protocols
WB protocol for SYNPO2L antibody 21480-1-APDownload protocol
IHC protocol for SYNPO2L antibody 21480-1-APDownload protocol
IF protocol for SYNPO2L antibody 21480-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols