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  • Featured Product
  • KD/KO Validated

SYT17 Polyclonal antibody

SYT17 Polyclonal Antibody for WB, IP, IF, FC, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, IF, FC, IHC, ELISA

Conjugate

Unconjugated

Cat no : 15413-1-AP

Synonyms

Protein B/K, Synaptotagmin 17, synaptotagmin XVII, SYT17, SytXVII

Validation Data Gallery

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  • Western Blot (WB) analysis of human kidney tissue using SYT17 Polyclonal antibody (15413-1-AP)

    WB analysis of human kidney using 15413-1-AP

    human kidney tissue were subjected to SDS PAGE followed by western blot with 15413-1-AP (SYT17 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of human testis tissue using SYT17 Polyclonal antibody (15413-1-AP)

    WB analysis of human testis using 15413-1-AP

    human testis tissue were subjected to SDS PAGE followed by western blot with 15413-1-AP (SYT17 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of mouse brain tissue using SYT17 Polyclonal antibody (15413-1-AP)

    WB analysis of mouse brain using 15413-1-AP

    mouse brain tissue were subjected to SDS PAGE followed by western blot with 15413-1-AP (SYT17 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of mouse brain tissue using SYT17 Polyclonal antibody (15413-1-AP)

    WB analysis of mouse brain using 15413-1-AP

    mouse brain tissue were subjected to SDS PAGE followed by western blot with 15413-1-AP (SYT17 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of A375 cells using SYT17 Polyclonal antibody (15413-1-AP)

    WB analysis of A375 using 15413-1-AP

    A375 cells were subjected to SDS PAGE followed by western blot with 15413-1-AP (SYT17 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of mouse lung tissue using SYT17 Polyclonal antibody (15413-1-AP)

    WB analysis of mouse lung using 15413-1-AP

    mouse lung tissue were subjected to SDS PAGE followed by western blot with 15413-1-AP (SYT17 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of human brain tissue using SYT17 Polyclonal antibody (15413-1-AP)

    WB analysis of human brain using 15413-1-AP

    human brain tissue were subjected to SDS PAGE followed by western blot with 15413-1-AP (SYT17 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of A375 cells using SYT17 Polyclonal antibody (15413-1-AP)

    WB analysis of A375 using 15413-1-AP

    A375 cells were subjected to SDS PAGE followed by western blot with 15413-1-AP (SYT17 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of PC-3 cells using SYT17 Polyclonal antibody (15413-1-AP)

    WB analysis of PC-3 using 15413-1-AP

    PC-3 cells were subjected to SDS PAGE followed by western blot with 15413-1-AP (SYT17 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of PC-3 cells using SYT17 Polyclonal antibody (15413-1-AP)

    WB analysis of PC-3 using 15413-1-AP

    PC-3 cells were subjected to SDS PAGE followed by western blot with 15413-1-AP (SYT17 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Immunoprecipitation (IP) experiment of mouse brain tissue using SYT17 Polyclonal antibody (15413-1-AP)

    IP experiment of mouse brain using 15413-1-AP

    IP result of anti-SYT17 (IP:15413-1-AP, 4ug; Detection:15413-1-AP 1:800) with mouse brain tissue lysate 6000ug.

  • Immunohistochemistry (IHC) staining of human brain tissue using SYT17 Polyclonal antibody (15413-1-AP)

    IHC staining of human brain using 15413-1-AP

    Immunohistochemical analysis of paraffin-embedded human brain using 15413-1-AP (SYT17 antibody) at dilution of 1:50 (under 40x lens).

  • Immunohistochemistry (IHC) staining of human lung cancer tissue using SYT17 Polyclonal antibody (15413-1-AP)

    IHC staining of human lung cancer using 15413-1-AP

    Immunohistochemical analysis of paraffin-embedded human lung cancer using 15413-1-AP (SYT17 antibody) at dilution of 1:50 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human lung cancer tissue using SYT17 Polyclonal antibody (15413-1-AP)

    IHC staining of human lung cancer using 15413-1-AP

    Immunohistochemical analysis of paraffin-embedded human lung cancer using 15413-1-AP (SYT17 antibody) at dilution of 1:50 (under 40x lens).

  • Immunohistochemistry (IHC) staining of human brain tissue using SYT17 Polyclonal antibody (15413-1-AP)

    IHC staining of human brain using 15413-1-AP

    Immunohistochemical analysis of paraffin-embedded human brain using 15413-1-AP (SYT17 antibody) at dilution of 1:50 (under 10x lens).

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using SYT17 Polyclonal antibody (15413-1-AP)

    IF Staining of HepG2 using 15413-1-AP

    Immunofluorescent analysis of HepG2 cells, using SYT17 antibody 15413-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).

  • Flow cytometry (FC) experiment of HepG2 cells using SYT17 Polyclonal antibody (15413-1-AP)

    FC experiment of HepG2 using 15413-1-AP

    1X10^6 HepG2 cells were stained with 0.2ug SYT17 antibody (15413-1-AP, red) and control antibody (blue). Fixed with 90% MeOH blocked with 3% BSA (30 min). Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) with dilution 1:1000.

Tested Applications

Positive WB detected inhuman kidney tissue, A375 cells, human brain tissue, human testis tissue, mouse brain tissue, mouse lung tissue, PC-3 cells
Positive IP detected inmouse brain tissue
Positive IHC detected inhuman brain tissue, human lung cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHepG2 cells
Positive FC detected inHepG2 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)IF : 1:10-1:100
Flow Cytometry (FC)FC : 0.20 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

15413-1-AP targets SYT17 in WB, IP, IF, FC, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen SYT17 fusion protein Ag7691
Full Name synaptotagmin XVII
Calculated Molecular Weight 54 kDa
Observed Molecular Weight 75-80 kDa
GenBank Accession NumberBC004518
Gene Symbol SYT17
Gene ID (NCBI) 51760
RRIDAB_2199457
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

The function of SYT17 has not been widely studied, and is yet to be fully elucidated. This antibody works well in WB, IHC and IF applications.

Protocols

Product Specific Protocols
WB protocol for SYT17 antibody 15413-1-APDownload protocol
IHC protocol for SYT17 antibody 15413-1-APDownload protocol
IF protocol for SYT17 antibody 15413-1-APDownload protocol
IP protocol for SYT17 antibody 15413-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanIHC,IF

Int Immunol

Increased urinary exosomal SYT17 levels in chronic active antibody-mediated rejection after kidney transplantation via the IL-6 amplifier.

Authors - Yusuke Takada
  • KD Validated
View Article