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Synaptotagmin-4 Polyclonal antibody

Synaptotagmin-4 Polyclonal Antibody for IHC, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 12642-1-AP

Synonyms

HsT1192, KIAA1342, Synaptotagmin 4, synaptotagmin IV, Synaptotagmin-4, SYT4, SytIV

Validation Data Gallery

Filter:
  • Western Blot (WB) analysis of human brain tissue using Synaptotagmin-4 Polyclonal antibody (12642-1-AP)

    WB analysis of human brain using 12642-1-AP

    human brain tissue were subjected to SDS PAGE followed by western blot with 12642-1-AP (Synaptotagmin-4 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of human brain tissue using Synaptotagmin-4 Polyclonal antibody (12642-1-AP)

    WB analysis of human brain using 12642-1-AP

    human brain tissue were subjected to SDS PAGE followed by western blot with 12642-1-AP (Synaptotagmin-4 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human gliomas tissue using Synaptotagmin-4 Polyclonal antibody (12642-1-AP)

    IHC staining of human gliomas using 12642-1-AP

    Immunohistochemical analysis of paraffin-embedded human gliomas using 12642-1-AP (Synaptotagmin-4 antibody) at dilution of 1:100 (under 10x lens).

Tested Applications

Positive WB detected inhuman brain tissue
Positive IHC detected inhuman gliomas tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

12642-1-AP targets Synaptotagmin-4 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Synaptotagmin-4 fusion protein Ag3338
Full Name synaptotagmin IV
Calculated Molecular Weight 425 aa, 48 kDa
Observed Molecular Weight 48 kDa
GenBank Accession NumberBC036538
Gene Symbol SYT4
Gene ID (NCBI) 6860
RRIDAB_2302982
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Protocols

Product Specific Protocols
WB protocol for Synaptotagmin-4 antibody 12642-1-APDownload protocol
IHC protocol for Synaptotagmin-4 antibody 12642-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanIHC

Histochem Cell Biol

Significance of achaete-scute complex homologue 1 (ASCL1) in pulmonary neuroendocrine carcinomas; RNA sequence analyses using small cell lung cancer cells and Ascl1-induced pulmonary neuroendocrine carcinoma cells.

Authors - Shinji Kudoh
View Article
humanWB

J Biol Chem

Lactate promotes neuronal differentiation of SH-SY5Y cells by lactate-responsive gene sets through NDRG3-dependent and -independent manners

Authors - Yidan Xu
View Article