TACC2 Polyclonal antibody
TACC2 Polyclonal Antibody for WB, IF, IHC, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse, rat
Applications
WB, IF, IHC, ELISA
Conjugate
Unconjugated
Cat no : 11407-1-AP
Synonyms
Validation Data Gallery
Tested Applications
| Positive WB detected in | mouse testis tissue |
| Positive IHC detected in | human lung cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF detected in | A431 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:4000 |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| Immunofluorescence (IF) | IF : 1:10-1:100 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| WB | See 2 publications below |
| IHC | See 1 publications below |
Product Information
11407-1-AP targets TACC2 in WB, IF, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | TACC2 fusion protein Ag1923 |
| Full Name | transforming, acidic coiled-coil containing protein 2 |
| Calculated Molecular Weight | 2948 aa, 309 kDa |
| Observed Molecular Weight | 73 kDa |
| GenBank Accession Number | BC015736 |
| Gene Symbol | TACC2 |
| Gene ID (NCBI) | 10579 |
| RRID | AB_2200456 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
Transforming acidic coiled-coil (TACC) proteins are a conserved family of centrosome- and microtubule-interacting proteins that are implicated in cancer. TACC2, also known as anti-zuai-1 (AZU-1), was abundantly expressed in non- and premalignant cells and tissues but was appreciably reduced in breast tumor cell types and in primary tumors, thus TACC2 may act as a tumor suppressor protein and represent a tumor progression marker. Several transcript variants encoding different isoforms have been found for this gene. Chromosome segregation in mitosis is orchestrated by dynamic interaction between spindle microtubule and the kinetochore. Centrosomal TACC2 plays a role in organizing centrosomal microtubules and is required for mitotic spindle maintenance.
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for TACC2 antibody 11407-1-AP | Download protocol |
| IHC protocol for TACC2 antibody 11407-1-AP | Download protocol |
| IF protocol for TACC2 antibody 11407-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Cancer Biomark High expression of TACC2 in hepatocellular carcinoma is associated with poor prognosis. | ||
F1000Res Androgen-dependent alternative mRNA isoform expression in prostate cancer cells. |
