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  • Featured Product
  • KD/KO Validated

TAF9 Polyclonal antibody

TAF9 Polyclonal Antibody for WB, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, IHC, ELISA

Conjugate

Unconjugated

Cat no : 10544-1-AP

Synonyms

AD 004, AK6, CGI 137, CINAP, CIP, STAF31/32, TAF2G, TAF9, TAFII 31, TAFII 32, TAFII31, TAFII32, TAFIID32

Validation Data Gallery

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  • Western Blot (WB) analysis of HeLa cells using TAF9 Polyclonal antibody (10544-1-AP)

    WB analysis of HeLa using 10544-1-AP

    HeLa cells were subjected to SDS PAGE followed by western blot with 10544-1-AP (TAF9 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of rat liver tissue using TAF9 Polyclonal antibody (10544-1-AP)

    WB analysis of rat liver using 10544-1-AP

    rat liver tissue were subjected to SDS PAGE followed by western blot with 10544-1-AP (TAF9 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HeLa cells using TAF9 Polyclonal antibody (10544-1-AP)

    WB analysis of HeLa using 10544-1-AP

    HeLa cells were subjected to SDS PAGE followed by western blot with 10544-1-AP (TAF9 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of MCF-7 cells using TAF9 Polyclonal antibody (10544-1-AP)

    WB analysis of MCF-7 using 10544-1-AP

    MCF-7 cells were subjected to SDS PAGE followed by western blot with 10544-1-AP (TAF9 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of human colon tissue using TAF9 Polyclonal antibody (10544-1-AP)

    WB analysis of human colon using 10544-1-AP

    human colon tissue were subjected to SDS PAGE followed by western blot with 10544-1-AP (TAF9 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of human skeletal muscle tissue using TAF9 Polyclonal antibody (10544-1-AP)

    WB analysis of human skeletal muscle using 10544-1-AP

    human skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 10544-1-AP (TAF9 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of human heart tissue using TAF9 Polyclonal antibody (10544-1-AP)

    WB analysis of human heart using 10544-1-AP

    human heart tissue were subjected to SDS PAGE followed by western blot with 10544-1-AP (TAF9 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of MCF-7 cells using TAF9 Polyclonal antibody (10544-1-AP)

    WB analysis of MCF-7 using 10544-1-AP

    MCF7 cells were subjected to SDS PAGE followed by western blot with 10544-1-AP (TAF9 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HEK-293 cells using TAF9 Polyclonal antibody (10544-1-AP)

    WB analysis of HEK-293 using 10544-1-AP

    HEK-293 cells were subjected to SDS PAGE followed by western blot with 10544-1-AP (TAF9 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Immunoprecipitation (IP) experiment of mouse skeletal muscle tissue using TAF9 Polyclonal antibody (10544-1-AP)

    IP experiment of mouse skeletal muscle using 10544-1-AP

    IP result of anti-TAF9 (IP:10544-1-AP, 4ug; Detection:10544-1-AP 1:1000) with mouse skeletal muscle tissue lysate 2200ug.

Tested Applications

Positive WB detected inHeLa cells, MCF-7 cells, human colon tissue, human skeletal muscle tissue, HEK-293 cells, human heart tissue, rat liver tissue
Positive IP detected inmouse skeletal muscle tissue

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

10544-1-AP targets TAF9 in WB, IP, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen TAF9 fusion protein Ag0833
Full Name TAF9 RNA polymerase II, TATA box binding protein (TBP)-associated factor, 32kDa
Calculated Molecular Weight 28 kDa
Observed Molecular Weight 24-28 kDa
GenBank Accession NumberBC007426
Gene Symbol TAF9
Gene ID (NCBI) 6880
RRIDAB_2271416
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

TAF9, also named as TAF2G, TAFII31, is a 264 amino acid protein, which belongs to the TAF9 family. TAF9 are involved in transcriptional activation as well as repression of distinct but overlapping sets of genes. TAF9 may have a role in gene regulation associated with apoptosis.

Protocols

Product Specific Protocols
WB protocol for TAF9 antibody 10544-1-APDownload protocol
IP protocol for TAF9 antibody 10544-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanIHC

Cancers (Basel)

Identification of Prognostic Markers of Gynecologic Cancers Utilizing Patient-Derived Xenograft Mouse Models.

Authors - Ha-Yeon Shin
View Article
humanWB

Front Oncol

Stable Isotope Tracing Uncovers Reduced γ/β-ATP Turnover and Metabolic Flux Through Mitochondrial-Linked Phosphotransfer Circuits in Aggressive Breast Cancer Cells.

Authors - Aleksandr Klepinin
View Article
mouse,ratWB,IHC,IP

Front Pharmacol

Activation of TAF9 via Danshensu-Induced Upregulation of HDAC1 Expression Alleviates Non-alcoholic Fatty Liver Disease.

Authors - Ruiwen Wang
  • KD Validated
View Article
humanWB

J Proteome Res

Targeted Proteomic Analysis Revealed Kinome Reprogramming during Acquisition of Radioresistance in Breast Cancer Cells.

Authors - Weili Miao
View Article