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TARSL2 Polyclonal antibody

TARSL2 Polyclonal Antibody for WB, IF, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IF, IHC, ELISA

Conjugate

Unconjugated

Cat no : 24635-1-AP

Synonyms

TARSL2, Threonine tRNA ligase, ThrRS

Validation Data Gallery

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  • Western Blot (WB) analysis of L02 cells using TARSL2 Polyclonal antibody (24635-1-AP)

    WB analysis of L02 using 24635-1-AP

    L02 cells were subjected to SDS PAGE followed by western blot with 24635-1-AP (TARSL2 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HEK-293 cells using TARSL2 Polyclonal antibody (24635-1-AP)

    WB analysis of HEK-293 using 24635-1-AP

    HEK-293 cells were subjected to SDS PAGE followed by western blot with 24635-1-AP (TARSL2 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human brain tissue using TARSL2 Polyclonal antibody (24635-1-AP)

    IHC staining of human brain using 24635-1-AP

    Immunohistochemical analysis of paraffin-embedded human brain tissue slide using 24635-1-AP (TARSL2 Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human brain tissue using TARSL2 Polyclonal antibody (24635-1-AP)

    IHC staining of human brain using 24635-1-AP

    Immunohistochemical analysis of paraffin-embedded human brain tissue slide using 24635-1-AP (TARSL2 Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunofluorescence (IF) / fluorescent staining of L02 cells using TARSL2 Polyclonal antibody (24635-1-AP)

    IF Staining of L02 using 24635-1-AP

    Immunofluorescent analysis of L02 cells using 24635-1-AP (TARSL2 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

Tested Applications

Positive WB detected inL02 cells, HEK-293 cells
Positive IHC detected inhuman brain tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inL02 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)IF : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

The immunogen of 24635-1-AP is TARSL2 Fusion Protein expressed in E. coli.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen TARSL2 fusion protein Ag18298
Full Name threonyl-tRNA synthetase-like 2
Calculated Molecular Weight 802 aa, 93 kDa
Observed Molecular Weight 93 kDa
GenBank Accession NumberBC132671
Gene Symbol TARSL2
Gene ID (NCBI) 123283
RRIDAB_2879648
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Protocols

Product Specific Protocols
WB protocol for TARSL2 antibody 24635-1-APDownload protocol
IHC protocol for TARSL2 antibody 24635-1-APDownload protocol
IF protocol for TARSL2 antibody 24635-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols