• Featured Product
  • KD/KO Validated

TBL1X Polyclonal antibody

TBL1X Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, IHC, CoIP, ChIP, ELISA

Conjugate

Unconjugated

Cat no : 13540-1-AP

Synonyms

EBI, SMAP55, TBL1, TBL1X



Tested Applications

Positive WB detected inmouse pancreas tissue
Positive IHC detected inmouse brain tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2400
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

13540-1-AP targets TBL1X in WB, IP, IHC, CoIP, ChIP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen TBL1X fusion protein Ag4477
Full Name transducin (beta)-like 1X-linked
Calculated Molecular Weight 526 aa, 57 kDa
Observed Molecular Weight 57 kDa
GenBank Accession NumberBC032708
Gene Symbol TBL1X
Gene ID (NCBI) 6907
RRIDAB_2199783
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

TBL1X is an essential subunit of the nuclear receptor corepressor (NCoR)-silencing mediator for retinoid and thyroid hormone receptors (SMRT) complex, the major TH receptor (TR) corepressor (CoR) involved in T3-regulated gene expression. TBL1X defects were recently associated with central hypothyroidism and hearing loss. (PMID: 30591955, PMID: 27603907)

Protocols

Product Specific Protocols
WB protocol for TBL1X antibody 13540-1-APDownload protocol
IHC protocol for TBL1X antibody 13540-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle

Development

Setd5 is essential for mammalian development and the co-transcriptional regulation of histone acetylation.

Authors - Anna B Osipovich
humanWB,IP

Int J Biol Sci

TBL1X and Flot2 form a positive feedback loop to promote metastasis in nasopharyngeal carcinoma.

Authors - Hongjuan Xu
  • KD Validated
mouseWB,CoIP,ChIP

Nat Commun

The Fgf/Erf/NCoR1/2 repressive axis controls trophoblast cell fate

Authors - Andreas Lackner
humanWB

Cell Chem Biol

HDAC3 and HDAC8 PROTAC dual degrader reveals roles of histone acetylation in gene regulation

Authors - Yufeng Xiao