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  • Featured Product
  • KD/KO Validated

TBRG4 Polyclonal antibody

TBRG4 Polyclonal Antibody for IF, IHC, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, IHC, IF, HITS-CLIP, ELISA

Conjugate

Unconjugated

Cat no : 16245-1-AP

Synonyms

CPR2, FASTKD4, KIAA0948, Protein TBRG4, TBRG4

Validation Data Gallery

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  • Western Blot (WB) analysis of HepG2 cells using TBRG4 Polyclonal antibody (16245-1-AP)

    WB analysis of HepG2 using 16245-1-AP

    HepG2 cells were subjected to SDS PAGE followed by western blot with 16245-1-AP (TBRG4 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of human liver tissue using TBRG4 Polyclonal antibody (16245-1-AP)

    WB analysis of human liver using 16245-1-AP

    human liver tissue were subjected to SDS PAGE followed by western blot with 16245-1-AP (TBRG4 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of mouse ovary tissue using TBRG4 Polyclonal antibody (16245-1-AP)

    WB analysis of mouse ovary using 16245-1-AP

    mouse ovary tissue were subjected to SDS PAGE followed by western blot with 16245-1-AP (TBRG4 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HeLa cells using TBRG4 Polyclonal antibody (16245-1-AP)

    WB analysis of HeLa using 16245-1-AP

    HeLa cells were subjected to SDS PAGE followed by western blot with 16245-1-AP (TBRG4 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Immunoprecipitation (IP) experiment of HepG2 cells using TBRG4 Polyclonal antibody (16245-1-AP)

    IP experiment of HepG2 using 16245-1-AP

    IP result of anti-TBRG4 (IP:16245-1-AP, 4ug; Detection:16245-1-AP 1:500) with HepG2 cells lysate 1600ug.

  • Immunohistochemistry (IHC) staining of human liver cancer tissue using TBRG4 Polyclonal antibody (16245-1-AP)

    IHC staining of human liver cancer using 16245-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 16245-1-AP (TBRG4 Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human liver cancer tissue using TBRG4 Polyclonal antibody (16245-1-AP)

    IHC staining of human liver cancer using 16245-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 16245-1-AP (TBRG4 Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunofluorescence (IF) / fluorescent staining of U2OS cells using TBRG4 Polyclonal antibody (16245-1-AP)

    IF Staining of U2OS cells using 16245-1-AP

    IF result of anti-TBRG4 (16245-1-AP) with U2OS cells.

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using TBRG4 Polyclonal antibody (16245-1-AP)

    IF Staining of HeLa using 16245-1-AP

    Immunofluorescent analysis of Hela cells, using TBRG4 antibody 16245-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).

  • Immunofluorescence (IF) / fluorescent staining of COS7 cells using TBRG4 Polyclonal antibody (16245-1-AP)

    IF Staining of COS7 cells using 16245-1-AP

    IF result of 16245-1-AP (anti-KD4) in COS7 cell by Dr. Nancy Kedersha. COS7 cells, fixed with 4% paraformaldehyde, post/fied and permeabilized with methanol, and stained with PTG anti-FAST KD4 antibody(16245-1-AP) in green, and human anti-mito autoimune sera from Immunovision in red. Nuclei are stained blue using Hoechst dye.

Tested Applications

Positive WB detected inHepG2 cells, HeLa cells, human liver tissue, mouse ovary tissue
Positive IP detected inHepG2 cells
Positive IHC detected inhuman liver cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inU2OS cells, Hela cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:3000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:100-1:400
Immunofluorescence (IF)IF : N/A
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

16245-1-AP targets TBRG4 in WB, IP, IHC, IF, HITS-CLIP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen TBRG4 fusion protein Ag9176
Full Name transforming growth factor beta regulator 4
Calculated Molecular Weight 631 aa, 71 kDa
Observed Molecular Weight 60 kDa, 71 kDa
GenBank Accession NumberBC017235
Gene Symbol TBRG4
Gene ID (NCBI) 9238
RRIDAB_2271487
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

TBRG4 is also named as CPR2(Cell cycle progression restoration protein 2), FASTKD4(FAST kinase domain-containing protein 4), KIAA0948 and belongs to the FAST kinase family. This protein contains multiple putative leucine zipper domains characteristic of transcription factors and it may play a role in cell cycle progression(PMID:9383053). RT-PCR ELISA detected moderate expression in ovary, and little to no expression in all other tissues and specific brain regions examined(PMID:10231032).TBRG4 has an mature form with MW of 60 kDa produced by transit peptide missing.

Protocols

Product Specific Protocols
WB protocol for TBRG4 antibody 16245-1-APDownload protocol
IHC protocol for TBRG4 antibody 16245-1-APDownload protocol
IF protocol for TBRG4 antibody 16245-1-APDownload protocol
IP protocol for TBRG4 antibody 16245-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB,HITS-CLIP

PLoS Genet

The FASTK family proteins fine-tune mitochondrial RNA processing

Authors - Akira Ohkubo
  • KO Validated
View Article
humanWB

Life Sci Alliance

Silica-based solid-phase extraction of cross-linked nucleic acid-bound proteins.

Authors - Claudio Asencio
View Article

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

FH

Erik (Verified Customer) (02-24-2021)

The antibody used at 1:500 dilution produces a clean and strong band at the expected height for the protein

  • Applications: Western Blot
  • Cell Tissue Type: PANC1 and MIAPACA2 cell lines
TBRG4 Antibody Western Blot validation ( dilution) in PANC1 and MIAPACA2 cell lines (Cat no:16245-1-AP)