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TIAF1 Polyclonal antibody

TIAF1 Polyclonal Antibody for WB, IF, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IF, IHC, ELISA

Conjugate

Unconjugated

Cat no : 20328-1-AP

Synonyms

MAJN, MYO18A, MYSPDZ, SPR210, TIAF1

Validation Data Gallery

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  • Western Blot (WB) analysis of PC-3 cells using TIAF1 Polyclonal antibody (20328-1-AP)

    WB analysis of PC-3 using 20328-1-AP

    PC-3 cells were subjected to SDS PAGE followed by western blot with 20328-1-AP (TIAF1 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of PC-3 cells using TIAF1 Polyclonal antibody (20328-1-AP)

    WB analysis of PC-3 using 20328-1-AP

    PC-3 cells were subjected to SDS PAGE followed by western blot with 20328-1-AP (TIAF1 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human skin tissue using TIAF1 Polyclonal antibody (20328-1-AP)

    IHC staining of human skin using 20328-1-AP

    Immunohistochemical analysis of paraffin-embedded human skin using 20328-1-AP (TIAF1 antibody) at dilution of 1:100 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human skin tissue using TIAF1 Polyclonal antibody (20328-1-AP)

    IHC staining of human skin using 20328-1-AP

    Immunohistochemical analysis of paraffin-embedded human skin using 20328-1-AP (TIAF1 antibody) at dilution of 1:100 (under 40x lens).

  • Immunofluorescence (IF) / fluorescent staining of PC-3 cells using TIAF1 Polyclonal antibody (20328-1-AP)

    IF Staining of PC-3 using 20328-1-AP

    Immunofluorescent analysis of (10% Formaldehyde) fixed PC-3 cells using 20328-1-AP (TIAF1 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

Tested Applications

Positive WB detected inPC-3 cells
Positive IHC detected inhuman skin tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inPC-3 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:3000
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)IF : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

WBSee 1 publications below

Product Information

The immunogen of 20328-1-AP is TIAF1 Fusion Protein expressed in E. coli.

Tested Reactivity human
Cited Reactivityhuman
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen TIAF1 fusion protein Ag14023
Full Name TGFB1-induced anti-apoptotic factor 1
Calculated Molecular Weight 115 aa, 12 kDa
Observed Molecular Weight 12 kDa
GenBank Accession NumberBC131561
Gene Symbol TIAF1
Gene ID (NCBI) 9220
RRIDAB_10858233
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Protocols

Product Specific Protocols
WB protocol for TIAF1 antibody 20328-1-APDownload protocol
IHC protocol for TIAF1 antibody 20328-1-APDownload protocol
IF protocol for TIAF1 antibody 20328-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Mol Carcinog

Upregulation of MGP by HOXC8 promotes the proliferation, migration, and EMT processes of triple-negative breast cancer.

Authors - Chen Gong
View Article