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TIMM10 Polyclonal antibody

TIMM10 Polyclonal Antibody for IHC, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC,ELISA

Conjugate

Unconjugated

Cat no : 11124-2-AP

Synonyms

TIM10, TIM10A, TIMM10

Validation Data Gallery

Filter:
  • Western Blot (WB) analysis of human heart tissue using TIMM10 Polyclonal antibody (11124-2-AP)

    WB analysis of human heart using 11124-2-AP

    human heart tissue were subjected to SDS PAGE followed by western blot with 11124-2-AP (TIMM10 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of human liver tissue using TIMM10 Polyclonal antibody (11124-2-AP)

    WB analysis of human liver using 11124-2-AP

    human liver tissue were subjected to SDS PAGE followed by western blot with 11124-2-AP (TIMM10 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human colon cancer tissue using TIMM10 Polyclonal antibody (11124-2-AP)

    IHC staining of human colon cancer using 11124-2-AP

    Immunohistochemical analysis of paraffin-embedded human colon cancer using 11124-2-AP (TIMM10 antibody) at dilution of 1:50 (under 25x lens).

Tested Applications

Positive WB detected inhuman heart tissue, human liver tissue
Positive IHC detected inhuman colon cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

WBSee 3 publications below

Product Information

11124-2-AP targets TIMM10 in WB, IHC,ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen TIMM10 fusion protein Ag1615
Full Name translocase of inner mitochondrial membrane 10 homolog (yeast)
Calculated Molecular Weight 10 kDa
Observed Molecular Weight 10 kDa
GenBank Accession NumberBC032133
Gene Symbol TIMM10
Gene ID (NCBI) 26519
RRIDAB_2201981
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Protocols

Product Specific Protocols
WB protocol for TIMM10 antibody 11124-2-APDownload protocol
IHC protocol for TIMM10 antibody 11124-2-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Cell Stem Cell

Disrupting Mitochondrial Copper Distribution Inhibits Leukemic Stem Cell Self-Renewal.

Authors - Rashim Pal Singh
View Article
ratWB

Redox Biol

Hypoxic acclimation improves cardiac redox homeostasis and protects heart against ischemia-reperfusion injury through upregulation of O-GlcNAcylation.

Authors - Wei Ou
View Article
humanWB

PLoS One

Megakaryocytic Differentiation of K562 Cells Induced by PMA Reduced the Activity of Respiratory Chain Complex IV.

Authors - Rui Huang
View Article