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TIMP-4 Polyclonal antibody

TIMP-4 Polyclonal Antibody for WB, IF, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human and More (1)

Applications

WB, IF, IHC, ELISA

Conjugate

Unconjugated

Cat no : 12326-1-AP

Synonyms

Metalloproteinase inhibitor 4, TIMP 4, TIMP4, TIMP-4

Validation Data Gallery

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  • Western Blot (WB) analysis of fetal human brain tissue using TIMP-4 Polyclonal antibody (12326-1-AP)

    WB analysis of fetal human brain using 12326-1-AP

    fetal human brain tissue were subjected to SDS PAGE followed by western blot with 12326-1-AP (TIMP-4 antibody at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of human heart tissue using TIMP-4 Polyclonal antibody (12326-1-AP)

    WB analysis of human heart using 12326-1-AP

    human heart tissue were subjected to SDS PAGE followed by western blot with 12326-1-AP (TIMP-4 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HT-1080 cells using TIMP-4 Polyclonal antibody (12326-1-AP)

    WB analysis of HT-1080 using 12326-1-AP

    HT-1080 cells were subjected to SDS PAGE followed by western blot with 12326-1-AP (TIMP-4 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of BxPC-3 cells using TIMP-4 Polyclonal antibody (12326-1-AP)

    WB analysis of BxPC-3 using 12326-1-AP

    BxPC-3 cells were subjected to SDS PAGE followed by western blot with 12326-1-AP (TIMP-4 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human gliomas tissue using TIMP-4 Polyclonal antibody (12326-1-AP)

    IHC staining of human gliomas using 12326-1-AP

    Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 12326-1-AP (TIMP-4 antibody at dilution of 1:50 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human gliomas tissue using TIMP-4 Polyclonal antibody (12326-1-AP)

    IHC staining of human gliomas using 12326-1-AP

    Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 12326-1-AP (TIMP-4 antibody at dilution of 1:50 (under 40x lens).

  • Immunohistochemistry (IHC) staining of human heart tissue using TIMP-4 Polyclonal antibody (12326-1-AP)

    IHC staining of human heart using 12326-1-AP

    Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 12326-1-AP (TIMP-4 antibody at dilution of 1:50 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human heart tissue using TIMP-4 Polyclonal antibody (12326-1-AP)

    IHC staining of human heart using 12326-1-AP

    Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 12326-1-AP (TIMP-4 antibody at dilution of 1:50 (under 40x lens).

  • Immunofluorescence (IF) / fluorescent staining of MCF-7 cells using TIMP-4 Polyclonal antibody (12326-1-AP)

    IF Staining of MCF-7 using 12326-1-AP

    Immunofluorescent analysis of MCF-7 cells using 12326-1-AP (TIMP-4 antibody) at dilution of 1:25 and Rhodamine-Goat anti-Rabbit IgG.

Tested Applications

Positive WB detected infetal human brain tissue, BxPC-3 cells, HT-1080 cells, human heart tissue
Positive IHC detected inhuman gliomas tissue, human heart tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inMCF-7 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)IF : 1:10-1:100
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

12326-1-AP targets TIMP-4 in WB, IF, IHC, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Cited Reactivityhuman, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen TIMP-4 fusion protein Ag2927
Full Name TIMP metallopeptidase inhibitor 4
Calculated Molecular Weight 224 aa, 26 kDa
Observed Molecular Weight 30 kDa
GenBank Accession NumberBC010553
Gene Symbol TIMP4
Gene ID (NCBI) 7079
RRIDAB_10638303
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Protocols

Product Specific Protocols
WB protocol for TIMP-4 antibody 12326-1-APDownload protocol
IHC protocol for TIMP-4 antibody 12326-1-APDownload protocol
IF protocol for TIMP-4 antibody 12326-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

J Med Chem

Discovery of a Novel Small-Molecule Inhibitor Disrupting TRBP-Dicer Interaction against Hepatocellular Carcinoma via the Modulation of microRNA Biogenesis.

Authors - Ting Peng
View Article
mouseWB,IF

Viruses

Coxsackievirus A2 Leads to Heart Injury in a Neonatal Mouse Model.

Authors - Wangquan Ji
View Article
humanWB

J Biochem Mol Toxicol

Pacenta polypeptide injection alleviates the fibrosis and inflammation in cigarette smoke extracts-induced BEAS-2B cells by modulating MMP-9/TIMP-1 signaling

Authors - Wei Xu
View Article
humanWB

Biochem Biophys Res Commun

Molecularly targeted anti-cancer drugs inhibit the invasion and metastasis of hepatocellular carcinoma by regulating the expression of MMP and TIMP gene families.

Authors - Xiao-Xiao He
View Article
mouseWB

PLoS One

Fructose-1,6-bisphosphate prevents pulmonary fibrosis by regulating extracellular matrix deposition and inducing phenotype reversal of lung myofibroblasts.

Authors - Henrique Bregolin Dias
View Article
humanWB

Biomed Res Int

Apatinib Inhibits the Invasion and Metastasis of Liver Cancer Cells by Downregulating MMP-Related Proteins via Regulation of the NF-κB Signaling Pathway.

Authors - Xiaoxiao He
View Article