• All
  • Primary Antibodies
  • Conjugated Antibodies for IF
  • Conjugated Antibodies for FC
  • Secondary Antibodies
  • Antibody Labeling KitsNew
  • ELISA Kits
  • IHC Kits
  • Magnetic Cell Separation Kits
  • Cytokines & Growth Factors
  • Neutralizing/activating Antibodies
  • Nanobody-based Reagents
  • Accessory Products and Kits
  • Fusion Proteins
×
×
Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0

Retrolinkin Polyclonal antibody

Retrolinkin Polyclonal Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, ELISA

Conjugate

Unconjugated

Cat no : 15177-1-AP

Synonyms

KIAA1690, Retrolinkin, RTLN, TMEM108, transmembrane protein 108

Validation Data Gallery

Filter:
  • Western Blot (WB) analysis of various lysates using Retrolinkin Polyclonal antibody (15177-1-AP)

    WB analysis using 15177-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 15177-1-AP (Retrolinkin antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HeLa cells using Retrolinkin Polyclonal antibody (15177-1-AP)

    WB analysis of HeLa using 15177-1-AP

    Hela cells were subjected to SDS PAGE followed by western blot with 15177-1-AP (Retrolinkin antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

Tested Applications

Positive WB detected inHeLa cells, Jurkat cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

15177-1-AP targets Retrolinkin in WB, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Retrolinkin fusion protein Ag7076
Full Name transmembrane protein 108
Calculated Molecular Weight 60 kDa
Observed Molecular Weight 55-60 kDa and 65-70 kDa
GenBank Accession NumberBC000568
Gene Symbol TMEM108
Gene ID (NCBI) 66000
RRIDAB_2204598
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Retrolinkin, also named as TMEM108, KIAA1690 and RTLN, is a membrane protein which plays an important role in retrograde axonal transport. It's highly enriched in neuronal endosomes, binds directly to BPAG1n4. This gene has two isoforms with calculated MW 60 kDa and 50 kDa. With modification, the MW of these two forms will be migrated to 65-70 kDa and 55-60 kDa.

Protocols

Product Specific Protocols
WB protocol for Retrolinkin antibody 15177-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols