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TMEM127 Polyclonal antibody

TMEM127 Polyclonal Antibody for IP, IF, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IP, IF, IHC, ELISA

Conjugate

Unconjugated

Cat no : 23142-1-AP

Synonyms

TMEM127, transmembrane protein 127

Validation Data Gallery

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  • Immunoprecipitation (IP) experiment of HeLa cells using TMEM127 Polyclonal antibody (23142-1-AP)

    IP experiment of HeLa using 23142-1-AP

    IP result of anti-TMEM127 (IP:23142-1-AP, 3ug; Detection:23142-1-AP 1:500) with HeLa cells lysate 3000ug.

  • Immunohistochemistry (IHC) staining of human heart tissue using TMEM127 Polyclonal antibody (23142-1-AP)

    IHC staining of human heart using 23142-1-AP

    Immunohistochemical analysis of paraffin-embedded human heart slide using 23142-1-AP (TMEM127 Antibody) at dilution of 1:50.

  • Immunohistochemistry (IHC) staining of human liver cancer tissue using TMEM127 Polyclonal antibody (23142-1-AP)

    IHC staining of human liver cancer using 23142-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver cancer slide using 23142-1-AP (TMEM127 Antibody) at dilution of 1:50 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human liver cancer tissue using TMEM127 Polyclonal antibody (23142-1-AP)

    IHC staining of human liver cancer using 23142-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver cancer slide using 23142-1-AP (TMEM127 Antibody) at dilution of 1:50 (under 40x lens).

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using TMEM127 Polyclonal antibody (23142-1-AP)

    IF Staining of HeLa using 23142-1-AP

    Immunofluorescent analysis of HeLa cells using 23142-1-AP (TMEM127 antibody) at dilution of 1:25 and Rhodamine-Goat anti-Rabbit IgG.

Tested Applications

Positive IP detected inHeLa cells
Positive IHC detected inhuman heart tissue, human liver cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHeLa cells

Recommended dilution

ApplicationDilution
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)IF : 1:10-1:100
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

WBSee 1 publications below

Product Information

The immunogen of 23142-1-AP is TMEM127 Fusion Protein expressed in E. coli.

Tested Reactivity human, mouse
Cited Reactivityhuman, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen TMEM127 fusion protein Ag19472
Full Name transmembrane protein 127
Calculated Molecular Weight 238 aa, 26 kDa
Observed Molecular Weight 26 kDa
GenBank Accession NumberBC039892
Gene Symbol TMEM127
Gene ID (NCBI) 55654
RRIDAB_2879217
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen Affinity purified
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Protocols

Product Specific Protocols
IHC protocol for TMEM127 antibody 23142-1-APDownload protocol
IF protocol for TMEM127 antibody 23142-1-APDownload protocol
IP protocol for TMEM127 antibody 23142-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
human,mouseWB

Elife

A cleaved METTL3 potentiates the METTL3-WTAP interaction and breast cancer progression

Authors - Chaojun Yan
View Article