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  • Featured Product
  • KD/KO Validated

TMEM132A Polyclonal antibody

TMEM132A Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 25301-1-AP

Synonyms

GBP, HSPA5 binding protein 1, HSPA5BP1, KIAA1583, TMEM132A, transmembrane protein 132A

Validation Data Gallery

Filter:
  • Western Blot (WB) analysis of various lysates using TMEM132A Polyclonal antibody (25301-1-AP)

    WB analysis using 25301-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 25301-1-AP (TMEM132A antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of fetal human brain tissue using TMEM132A Polyclonal antibody (25301-1-AP)

    WB analysis of fetal human brain using 25301-1-AP

    fetal human brain tissue were subjected to SDS PAGE followed by western blot with 25301-1-AP (TMEM132A Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of mouse brain tissue using TMEM132A Polyclonal antibody (25301-1-AP)

    IHC staining of mouse brain using 25301-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 25301-1-AP (TMEM132A antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

Tested Applications

Positive WB detected inHaCaT cells, fetal human brain tissue, MCF-7 cells, SH-SY5Y cells, U-87 MG cells, mouse brain tissue, rat brain tissue
Positive IHC detected inmouse brain tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:10000
Immunohistochemistry (IHC)IHC : 1:250-1:1000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

25301-1-AP targets TMEM132A in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen TMEM132A fusion protein Ag18142
Full Name transmembrane protein 132A
Calculated Molecular Weight 1023 aa, 110 kDa
Observed Molecular Weight 100-140 kDa
GenBank Accession NumberBC114569
Gene Symbol TMEM132A
Gene ID (NCBI) 54972
RRIDAB_2880018
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Protocols

Product Specific Protocols
WB protocol for TMEM132A antibody 25301-1-APDownload protocol
IHC protocol for TMEM132A antibody 25301-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
human,mouseWB

Front Cell Dev Biol

TMEM132A, a Novel Wnt Signaling Pathway Regulator Through Wntless (WLS) Interaction.

Authors - Binbin Li
  • KO Validated
View Article
human,mouseWB

Development

TMEM132A ensures mouse caudal neural tube closure and regulates integrin-based mesodermal migration

Authors - Binbin Li
  • KO Validated
View Article