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- KD/KO Validated
TMEM175 Polyclonal antibody
TMEM175 Polyclonal Antibody for WB, IHC, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse, rat and More (1)
Applications
WB, IP, IF, IHC, CoIP, ELISA
Conjugate
Unconjugated
Cat no : 19925-1-AP
Synonyms
Validation Data Gallery
Tested Applications
| Positive WB detected in | HeLa cells, HEK-293 cells, SH-SY5Y cells, HepG2 cells |
| Positive IHC detected in | mouse brain tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:3000 |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| KD/KO | See 4 publications below |
| WB | See 12 publications below |
| IF | See 1 publications below |
| IP | See 1 publications below |
| CoIP | See 1 publications below |
Product Information
19925-1-AP targets TMEM175 in WB, IP, IF, IHC, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse, rat, monkey |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | TMEM175 fusion protein Ag13890 |
| Full Name | transmembrane protein 175 |
| Calculated Molecular Weight | 504 aa, 56 kDa |
| Observed Molecular Weight | 41-45 kDa, 54-60 kDa |
| GenBank Accession Number | BC005158 |
| Gene Symbol | TMEM175 |
| Gene ID (NCBI) | 84286 |
| RRID | AB_10666166 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
TMEM175 has two repeats of 6-transmembrane-spanning segments and has no GYG K+ channel sequence signature-containing, pore-forming P loop. Lysosomes lacking TMEM175 exhibit no K+conductance, have a markedly depolarized ΔΨ and little sensitivity to changes in [K+], and have compromised luminal pH stability and abnormal fusion with autophagosomes during autophagy. TMEM175 comprises a K+ channel that underlies the molecular mechanism of lysosomal K+ permeability. It has two isoforms with MW 41-45 kDa and 54-60 kDa. For optimal WB detection with this antibody, we recommend avoiding boiling the sample after lysis.
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for TMEM175 antibody 19925-1-AP | Download protocol |
| IHC protocol for TMEM175 antibody 19925-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Nature A growth-factor-activated lysosomal K+ channel regulates Parkinson's pathology.
| ||
Ann Neurol Genetic, Structural, and Functional Evidence Link TMEM175 to Synucleinopathies. | ||
Proc Natl Acad Sci U S A TMEM175 deficiency impairs lysosomal and mitochondrial function and increases α-synuclein aggregation.
| ||
Mol Brain TMEM175 mediates Lysosomal function and participates in neuronal injury induced by cerebral ischemia-reperfusion. | ||
Hum Mol Genet Functionalization of the TMEM175 p.M393T Variant as a risk factor for Parkinson Disease.
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