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TMEM181 Polyclonal antibody

TMEM181 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 55411-1-AP

Synonyms

GPR178, KIAA1423, TMEM181, transmembrane protein 181

Validation Data Gallery

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  • Western Blot (WB) analysis of A431 cells using TMEM181 Polyclonal antibody (55411-1-AP)

    WB analysis of A431 using 55411-1-AP

    A431 cells were subjected to SDS PAGE followed by western blot with 55411-1-AP (TMEM181 antibody) at dilution of 1:200 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of mouse heart tissue using TMEM181 Polyclonal antibody (55411-1-AP)

    IHC staining of mouse heart using 55411-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 55411-1-AP (TMEM181 Antibody) at dilution of 1:200 (under 40x lens).

Tested Applications

Positive WB detected inA431 cells
Positive IHC detected inmouse heart tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:200-1:1000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

55411-1-AP targets TMEM181 in WB, IHC, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Peptide
Full Name transmembrane protein 181
Calculated Molecular Weight 69 kDa
Observed Molecular Weight 75 kDa
GenBank Accession NumberNM_020823
Gene Symbol TMEM181
Gene ID (NCBI) 57583
RRIDAB_11182284
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

TMEM181, also named as GPR178, KIAA1423, is a membrane protein. It mediates action of cytolethal distending toxins (CDT), which are secreted by many pathogenic bacteria. Expression level of TMEM181 is rate-limiting for intoxication. This antibody is specific to TMEM181.

Protocols

Product Specific Protocols
WB protocol for TMEM181 antibody 55411-1-APDownload protocol
IHC protocol for TMEM181 antibody 55411-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols