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TMEM27 Polyclonal antibody

TMEM27 Polyclonal Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB,ELISA

Conjugate

Unconjugated

Cat no : 14571-1-AP

Synonyms

Collectrin, TMEM27

Validation Data Gallery

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  • Western Blot (WB) analysis of mouse pancreas tissue using TMEM27 Polyclonal antibody (14571-1-AP)

    WB analysis of mouse pancreas using 14571-1-AP

    mouse pancreas tissue were subjected to SDS PAGE followed by western blot with 14571-1-AP (TMEM27 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of MCF-7 cells using TMEM27 Polyclonal antibody (14571-1-AP)

    WB analysis of MCF-7 using 14571-1-AP

    MCF7 cells were subjected to SDS PAGE followed by western blot with 14571-1-AP (TMEM27 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of human spleen tissue using TMEM27 Polyclonal antibody (14571-1-AP)

    WB analysis of human spleen using 14571-1-AP

    human spleen tissue were subjected to SDS PAGE followed by western blot with 14571-1-AP (TMEM27 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of mouse kidney tissue using TMEM27 Polyclonal antibody (14571-1-AP)

    WB analysis of mouse kidney using 14571-1-AP

    mouse kidney tissue were subjected to SDS PAGE followed by western blot with 14571-1-AP (TMEM27 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

Tested Applications

Positive WB detected inmouse pancreas tissue, human spleen tissue, MCF-7 cells, mouse kidney tissue

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

14571-1-AP targets TMEM27 in WB,ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen TMEM27 fusion protein Ag6187
Full Name transmembrane protein 27
Calculated Molecular Weight 25 kDa
Observed Molecular Weight 20 kDa
GenBank Accession NumberBC050606
Gene Symbol TMEM27
Gene ID (NCBI) 57393
RRIDAB_2204913
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

TMEM27 (also termed collectrin), a 46 kDa type I transmembrane protein, is a homolog of the noncatalytic domain of angiotensin-converting enzyme-related carboxypeptidase (Ace2). Its expression has only been reported in the brush border membrane of the proximal tubules and collecting ducts of the kidney and in the pancreatic b cell (PMID: 11278314, 16330324). Overexpression of TMEM27 in b cells leads to increased proliferation in vitro and increased pancreatic b cell mass in vivo, and also has been reported to augment glucosestimulated INS secretion (GSIS) (PMID: 16330324, 16330323). The abundance of TMEM27 protein in b cells is furthermore regulated by ectodomain cleavage, which leads to two cleavage products, a 25 kDa N-terminal part that is released into the extracellular space (the shed fragment), and a 22 kDa C-terminal fragment (CTF) remaining in the membrane that is rapidly degraded (PMID: 16330324). TMEM27 is an N-glycosylated protein and can form dimers with MW of 64-75 kDa(patent US 20100119489 A1). TMEM27 can be used as a beta cell mass biomarker (PMID: 20386877, 21907142).

Protocols

Product Specific Protocols
WB protocol for TMEM27 antibody 14571-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols