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TNFAIP1 Monoclonal antibody

TNFAIP1 Monoclonal Antibody for WB, IHC, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

human

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

CloneNo.

4F11B5

Cat no : 60327-1-Ig

Synonyms

B12, B61, BACURD2, EDP1, TNFAIP1

Validation Data Gallery

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  • Western Blot (WB) analysis of HUVEC cells using TNFAIP1 Monoclonal antibody (60327-1-Ig)

    WB analysis of HUVEC using 60327-1-Ig

    HUVEC, HEK-293 and HeLa cells were subjected to SDS PAGE followed by western blot with 60327-1-Ig (TNFAIP1 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HeLa cells using TNFAIP1 Monoclonal antibody (60327-1-Ig)

    WB analysis of HeLa using 60327-1-Ig

    HeLa cells were subjected to SDS PAGE followed by western blot with 60327-1-Ig (TNFAIP1 Antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human lung cancer tissue using TNFAIP1 Monoclonal antibody (60327-1-Ig)

    IHC staining of human lung cancer using 60327-1-Ig

    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 60327-1-Ig (TNFAIP1 antibody) at dilution of 1:300 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human lung cancer tissue using TNFAIP1 Monoclonal antibody (60327-1-Ig)

    IHC staining of human lung cancer using 60327-1-Ig

    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 60327-1-Ig (TNFAIP1 antibody) at dilution of 1:300 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human colon cancer tissue using TNFAIP1 Monoclonal antibody (60327-1-Ig)

    IHC staining of human colon cancer using 60327-1-Ig

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 60327-1-Ig (TNFAIP1 antibody) at dilution of 1:300 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human colon cancer tissue using TNFAIP1 Monoclonal antibody (60327-1-Ig)

    IHC staining of human colon cancer using 60327-1-Ig

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 60327-1-Ig (TNFAIP1 antibody) at dilution of 1:300 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

Tested Applications

Positive WB detected inHUVEC cells, HeLa cells
Positive IHC detected inhuman lung cancer tissue, human colon cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:6000
Immunohistochemistry (IHC)IHC : 1:150-1:600
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

WBSee 1 publications below

Product Information

60327-1-Ig targets TNFAIP1 in WB, IHC, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Cited Reactivityhuman
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen TNFAIP1 fusion protein Ag7873
Full Name tumor necrosis factor, alpha-induced protein 1 (endothelial)
Calculated Molecular Weight 36 kDa
Observed Molecular Weight 36 kDa
GenBank Accession NumberBC001949
Gene Symbol TNFAIP1
Gene ID (NCBI) 7126
RRIDAB_2881437
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Protocols

Product Specific Protocols
WB protocol for TNFAIP1 antibody 60327-1-IgDownload protocol
IHC protocol for TNFAIP1 antibody 60327-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Oncotarget

PMID: 29137317

Authors - Wu Shuang
View Article