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  • Featured Product
  • KD/KO Validated

TNKS Polyclonal antibody

TNKS Polyclonal Antibody for IF, IHC, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, IHC, IF, ELISA

Conjugate

Unconjugated

Cat no : 18030-1-AP

Synonyms

PARP 5a, PARP5A, PARPL, TANK1, Tankyrase 1, Tankyrase I, TIN1, TINF1, TNKS, TNKS 1, TNKS1

Validation Data Gallery

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  • Western Blot (WB) analysis of HeLa cells using TNKS Polyclonal antibody (18030-1-AP)

    WB analysis of HeLa using 18030-1-AP

    HeLa cells were subjected to SDS PAGE followed by western blot with 18030-1-AP (TNKS antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of Jurkat cells using TNKS Polyclonal antibody (18030-1-AP)

    WB analysis of Jurkat using 18030-1-AP

    Jurkat cells were subjected to SDS PAGE followed by western blot with 18030-1-AP (TNKS antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of MCF-7 cells using TNKS Polyclonal antibody (18030-1-AP)

    WB analysis of MCF-7 using 18030-1-AP

    MCF-7 cells were subjected to SDS PAGE followed by western blot with 18030-1-AP (TNKS antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Immunoprecipitation (IP) experiment of Jurkat cells using TNKS Polyclonal antibody (18030-1-AP)

    IP experiment of Jurkat using 18030-1-AP

    IP result of anti-TNKS (IP:18030-1-AP, 4ug; Detection:18030-1-AP 1:500) with Jurkat cells lysate 3200ug.

  • Immunohistochemistry (IHC) staining of mouse testis tissue using TNKS Polyclonal antibody (18030-1-AP)

    IHC staining of mouse testis using 18030-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 18030-1-AP (TNKS antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human colon cancer tissue using TNKS Polyclonal antibody (18030-1-AP)

    IHC staining of human colon cancer using 18030-1-AP

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 18030-1-AP (TNKS antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of mouse testis tissue using TNKS Polyclonal antibody (18030-1-AP)

    IHC staining of mouse testis using 18030-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 18030-1-AP (TNKS antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using TNKS Polyclonal antibody (18030-1-AP)

    IF Staining of HepG2 using 18030-1-AP

    Immunofluorescent analysis of HepG2 cells, using TNKS antibody 18030-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red). Blue pseudocolor = DAPI (fluorescent DNA dye).

Tested Applications

Positive WB detected inHeLa cells, Jurkat cells, MCF-7 cells
Positive IP detected inJurkat cells
Positive IHC detected inmouse testis tissue, human colon cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHepG2 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)IF : 1:10-1:100
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

18030-1-AP targets TNKS in WB, IP, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen TNKS fusion protein Ag12597
Full Name tankyrase, TRF1-interacting ankyrin-related ADP-ribose polymerase
Calculated Molecular Weight 1327 aa, 142 kDa
Observed Molecular Weight 150 kDa
GenBank Accession NumberBC098394
Gene Symbol TNKS
Gene ID (NCBI) 8658
RRIDAB_2303564
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Protocols

Product Specific Protocols
WB protocol for TNKS antibody 18030-1-APDownload protocol
IHC protocol for TNKS antibody 18030-1-APDownload protocol
IF protocol for TNKS antibody 18030-1-APDownload protocol
IP protocol for TNKS antibody 18030-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
WB

Mol Cell

PARP5A and RNF146 phase separation restrains RIPK1-dependent necroptosis

Authors - Shouqiao Hou
View Article

J Biol Chem

The dePARylase NUDT16 promotes radiation resistance of cancer cells by blocking SETD3 for degradation via reversing its ADP-ribosylation

Authors - Weijun Wu
View Article
humanIHC, WB, IF

Oncol Lett

Dihydroartemisinin suppresses proliferation, migration, the Wnt/β-catenin pathway and EMT via TNKS in gastric cancer.

Authors - Yanmei Ma
View Article
humanWB,IHC

Cell Death Dis

circ5615 functions as a ceRNA to promote colorectal cancer progression by upregulating TNKS.

Authors - Zhifei Ma
  • KD Validated
View Article
humanWB

Adv Sci (Weinh)

Transferrin Receptor-Mediated Iron Uptake Promotes Colon Tumorigenesis

Authors - Hyeoncheol Kim
View Article
humanWB

Chem Biol Interact

Identification and evaluation of a novel PARP1 inhibitor for the treatment of triple-negative breast cancer

Authors - Rong Gong
View Article