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  • Featured Product
  • KD/KO Validated

TNS4 Polyclonal antibody

TNS4 Polyclonal Antibody for WB, IF, FC, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IF, FC, IHC, CoIP, ELISA, Cell treatment

Conjugate

Unconjugated

Cat no : 11580-1-AP

Synonyms

C terminal tensin like protein, CTEN, PP14434, tensin 4, TNS4

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using TNS4 Polyclonal antibody (11580-1-AP)

    WB analysis using 11580-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 11580-1-AP (TNS4 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of human placenta tissue using TNS4 Polyclonal antibody (11580-1-AP)

    WB analysis of human placenta using 11580-1-AP

    human placenta tissue were subjected to SDS PAGE followed by western blot with 11580-1-AP (TNS4 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human liver cancer tissue using TNS4 Polyclonal antibody (11580-1-AP)

    IHC staining of human liver cancer using 11580-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 11580-1-AP (TNS4 Antibody) at dilution of 1:50 (under 40x lens).

  • Immunohistochemistry (IHC) staining of human liver cancer tissue using TNS4 Polyclonal antibody (11580-1-AP)

    IHC staining of human liver cancer using 11580-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 11580-1-AP (TNS4 Antibody) at dilution of 1:50 (under 10x lens).

  • Immunofluorescence (IF) / fluorescent staining of A549 cells using TNS4 Polyclonal antibody (11580-1-AP)

    IF Staining of A549 using 11580-1-AP

    Immunofluorescent analysis of (-20°C Ethanol) fixed A549 cells using 11580-1-AP (TNS4 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

  • Flow cytometry (FC) experiment of HepG2 cells using TNS4 Polyclonal antibody (11580-1-AP)

    FC experiment of HepG2 using 11580-1-AP

    1X10^6 HepG2 cells were stained with 0.2ug TNS4 antibody (11580-1-AP, red) and control antibody (blue). Fixed with 90% MeOH blocked with 3% BSA (30 min). Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) with dilution 1:1000.

Tested Applications

Positive WB detected inHepG2 cells, human placenta tissue, PC-12 cells, NIH/3T3 cells, RAW 264.7 cells
Positive IHC detected inhuman liver cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inA549 cells
Positive FC detected inHepG2 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)IF : 1:50-1:500
Flow Cytometry (FC)FC : 0.20 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

11580-1-AP targets TNS4 in WB, IF, FC, IHC, CoIP, ELISA, Cell treatment applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen TNS4 fusion protein Ag2169
Full Name tensin 4
Calculated Molecular Weight 715 aa, 77 kDa
Observed Molecular Weight 77 kDa
GenBank Accession NumberBC018706
Gene Symbol TNS4
Gene ID (NCBI) 84951
RRIDAB_10640582
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Protocols

Product Specific Protocols
WB protocol for TNS4 antibody 11580-1-APDownload protocol
IHC protocol for TNS4 antibody 11580-1-APDownload protocol
IF protocol for TNS4 antibody 11580-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouseIF

Front Bioeng Biotechnol

Smarce1 and Tensin 4 Are Putative Modulators of Corneoscleral Stiffness.

Authors - Elizabeth M Boazak
View Article
humanWB

Pathol Res Pract

Tensin4 promotes invasion and migration of gastric cancer cells via regulating AKT/GSK-3β/snail signaling pathway.

Authors - Xiumin Qi
  • KD Validated
View Article
humanWB

Antioxid Redox Signal

A novel lncRNA FUAT1/TNS4 axis confers chemoresistance by suppressing ROS-mediated apoptosis in gastric cancer

Authors - Mingliang Liu
View Article
human,mouseCell treatment,IHC,WB,CoIP

Int J Biol Sci

Hypoxia-driven TNS4 fosters HNSCC tumorigenesis by stabilizing integrin α5β1 complex and triggering FAK-mediated Akt and TGFβ signaling pathways

Authors - Xinyuan Zhao
View Article