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TOR1AIP1 Polyclonal antibody

TOR1AIP1 Polyclonal Antibody for WB, IF, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IF, ELISA

Conjugate

Unconjugated

Cat no : 21459-1-AP

Synonyms

LAP1B, TOR1AIP1, torsin A interacting protein 1

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using TOR1AIP1 Polyclonal antibody (21459-1-AP)

    WB analysis using 21459-1-AP

    COLO 320 cells were subjected to SDS PAGE followed by western blot with 21459-1-AP (TOR1AIP1 antibody) at dilution of 1:15000 incubated at room temperature for 1.5 hours.

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using TOR1AIP1 Polyclonal antibody (21459-1-AP)

    IF Staining of HeLa using 21459-1-AP

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using TOR1AIP1 antibody (21459-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red).

Tested Applications

Positive WB detected inCOLO 320 cells, HeLa cells, HEK-293T cells
Positive IF detected inHeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunofluorescence (IF)IF : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

21459-1-AP targets TOR1AIP1 in WB, IF, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Cited Reactivityhuman
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen TOR1AIP1 fusion protein Ag14823
Full Name torsin A interacting protein 1
Calculated Molecular Weight462aa,52 kDa; 583aa,66 kDa
Observed Molecular Weight 50-55 kDa
GenBank Accession NumberBC023247
Gene Symbol TOR1AIP1
Gene ID (NCBI) 26092
RRIDAB_2878861
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Protocols

Product Specific Protocols
WB protocol for TOR1AIP1 antibody 21459-1-APDownload protocol
IF protocol for TOR1AIP1 antibody 21459-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB,IF

Elife

CDK1 controls CHMP7-dependent nuclear envelope reformation.

Authors - Alberto T Gatta
View Article
humanWB,IF

Nat Cell Biol

LAP1 supports nuclear adaptability during constrained melanoma cell migration and invasion

Authors - Yaiza Jung-Garcia
View Article