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TRAF3IP1 Polyclonal antibody

TRAF3IP1 Polyclonal Antibody for WB, IP, IF, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, IF, IHC, ELISA

Conjugate

Unconjugated

Cat no : 14404-1-AP

Synonyms

MIP T3, MIPT3, TRAF3 interacting protein 1, TRAF3IP1

Validation Data Gallery

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  • Western Blot (WB) analysis of HEK-293 cells using TRAF3IP1 Polyclonal antibody (14404-1-AP)

    WB analysis of HEK-293 using 14404-1-AP

    HEK-293 cells were subjected to SDS PAGE followed by western blot with 14404-1-AP (TRAF3IP1 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • Immunoprecipitation (IP) experiment of HeLa cells using TRAF3IP1 Polyclonal antibody (14404-1-AP)

    IP experiment of HeLa using 14404-1-AP

    IP result of anti-TRAF3IP1 (IP:14404-1-AP, 3ug; Detection:14404-1-AP 1:300) with HeLa cells lysate 2000ug.

  • Immunohistochemistry (IHC) staining of human breast cancer tissue using TRAF3IP1 Polyclonal antibody (14404-1-AP)

    IHC staining of human breast cancer using 14404-1-AP

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 14404-1-AP (TRAF3IP1 Antibody) at dilution of 1:50 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human breast cancer tissue using TRAF3IP1 Polyclonal antibody (14404-1-AP)

    IHC staining of human breast cancer using 14404-1-AP

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 14404-1-AP (TRAF3IP1 Antibody) at dilution of 1:50 (under 40x lens).

  • Immunohistochemistry (IHC) staining of human cervical cancer tissue using TRAF3IP1 Polyclonal antibody (14404-1-AP)

    IHC staining of human cervical cancer using 14404-1-AP

    Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue slide using 14404-1-AP (TRAF3IP1 Antibody) at dilution of 1:50 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human cervical cancer tissue using TRAF3IP1 Polyclonal antibody (14404-1-AP)

    IHC staining of human cervical cancer using 14404-1-AP

    Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue slide using 14404-1-AP (TRAF3IP1 Antibody) at dilution of 1:50 (under 40x lens).

  • Immunofluorescence (IF) / fluorescent staining of NIH/3T3 cells using TRAF3IP1 Polyclonal antibody (14404-1-AP)

    IF Staining of NIH/3T3 using 14404-1-AP

    Immunofluorescent analysis of NIH/3T3 cells using 14404-1-AP (TRAF3IP1 antibody) at dilution of 1:25 and Rhodamine-Goat anti-Rabbit IgG.

Tested Applications

Positive WB detected inHEK-293 cells
Positive IP detected inHeLa cells
Positive IHC detected inhuman breast cancer tissue, human cervical cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inNIH/3T3 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:200-1:1000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)IF : 1:10-1:100
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

14404-1-AP targets TRAF3IP1 in WB, IP, IF, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen TRAF3IP1 fusion protein Ag5677
Full Name TNF receptor-associated factor 3 interacting protein 1
Calculated Molecular Weight 79 kDa
Observed Molecular Weight 83 kDa
GenBank Accession NumberBC059174
Gene Symbol TRAF3IP1
Gene ID (NCBI) 26146
RRIDAB_2878054
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

TRAF3IP1, also known as MIPT3, was initially characterized through its interactions with tubulin, actin, TNFR-associated factor-3 (Traf3), IL-13R1, and DISC1. It functions as an inhibitor of IL-13-mediated phosphorylation of Stat6 and in sequestration of Traf3 and DISC1 to the cytoskeleton.

Protocols

Product Specific Protocols
WB protocol for TRAF3IP1 antibody 14404-1-APDownload protocol
IHC protocol for TRAF3IP1 antibody 14404-1-APDownload protocol
IF protocol for TRAF3IP1 antibody 14404-1-APDownload protocol
IP protocol for TRAF3IP1 antibody 14404-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols