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TRAPPC2 Polyclonal antibody

TRAPPC2 Polyclonal Antibody for IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse and More (1)

Applications

WB,IP,ELISA

Conjugate

Unconjugated

Cat no : 12484-1-AP

Synonyms

hYP38334, MBP 1 interacting protein 2A, MIP 2A, SEDL, Sedlin, SEDLP, SEDT, TRAPPC2, TRAPPC2P1, TRS20, ZNF547L

Validation Data Gallery

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  • Western Blot (WB) analysis of human brain tissue using TRAPPC2 Polyclonal antibody (12484-1-AP)

    WB analysis of human brain using 12484-1-AP

    human brain tissue were subjected to SDS PAGE followed by western blot with 12484-1-AP (TRAPPC2 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of PC-3 cells using TRAPPC2 Polyclonal antibody (12484-1-AP)

    WB analysis of PC-3 using 12484-1-AP

    PC-3 cells were subjected to SDS PAGE followed by western blot with 12484-1-AP (TRAPPC2 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of human liver tissue using TRAPPC2 Polyclonal antibody (12484-1-AP)

    WB analysis of human liver using 12484-1-AP

    human liver tissue were subjected to SDS PAGE followed by western blot with 12484-1-AP (TRAPPC2 antibody) at dilution of 1:1200 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of human lung tissue using TRAPPC2 Polyclonal antibody (12484-1-AP)

    WB analysis of human lung using 12484-1-AP

    human lung tissue were subjected to SDS PAGE followed by western blot with 12484-1-AP (TRAPPC2 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of mouse thymus tissue using TRAPPC2 Polyclonal antibody (12484-1-AP)

    WB analysis of mouse thymus using 12484-1-AP

    mouse thymus tissue were subjected to SDS PAGE followed by western blot with 12484-1-AP (TRAPPC2 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of mouse brain tissue using TRAPPC2 Polyclonal antibody (12484-1-AP)

    WB analysis of mouse brain using 12484-1-AP

    mouse brain tissue were subjected to SDS PAGE followed by western blot with 12484-1-AP (TRAPPC2 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • Immunoprecipitation (IP) experiment of PC-3 cells using TRAPPC2 Polyclonal antibody (12484-1-AP)

    IP experiment of PC-3 using 12484-1-AP

    IP result of anti-TRAPPC2 (IP:12484-1-AP, 3ug; Detection:12484-1-AP 1:500) with PC-3 cells lysate 2800ug.

Tested Applications

Positive WB detected inhuman brain tissue, human liver tissue, human lung tissue, mouse brain tissue, mouse thymus tissue, PC-3 cells
Positive IP detected inPC-3 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2400
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

WBSee 3 publications below

Product Information

12484-1-AP targets TRAPPC2 in WB,IP,ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Cited Reactivityhuman, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen TRAPPC2 fusion protein Ag3201
Full Name trafficking protein particle complex 2
Calculated Molecular Weight 140 aa, 16 kDa
Observed Molecular Weight 16 kDa
GenBank Accession NumberBC016915
Gene Symbol TRAPPC2
Gene ID (NCBI) 6399
RRIDAB_2208136
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

TRAPPC2 is one component of the TRAPP multisubunit tethering complex involved in intracellular vesicle trafficking [PMID:21525244]. It acts as an adaptor for the TRAPP complex in mammalian cells, mediating interactions with both TRAPPC9 and TRAPPC8. It prevents transcriptional repression and induction of cell death by ENO1. Also it plays a role in vesicular transport from endoplasmic reticulum to Golgi.

Protocols

Product Specific Protocols
WB protocol for TRAPPC2 antibody 12484-1-APDownload protocol
IP protocol for TRAPPC2 antibody 12484-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Proc Natl Acad Sci U S A

Primary cilia membrane assembly is initiated by Rab11 and transport protein particle II (TRAPPII) complex-dependent trafficking of Rabin8 to the centrosome.

Authors - Westlake Christopher J CJ
View Article
humanWB

J Biol Chem

The C7orf43/TRAPPC14 component links the TRAPPII complex to RABIN8 for preciliary vesicle tethering at the mother centriole during ciliogenesis.

Authors - Adrian Cuenca
View Article
ratWB

iScience

Mea6/cTAGE5 cooperates with TRAPPC12 to regulate PTN secretion and white matter development

Authors - Tiantian Ma
View Article