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TRAPPC4 Polyclonal antibody

TRAPPC4 Polyclonal Antibody for IF, IHC, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, IHC, IF, ELISA

Conjugate

Unconjugated

Cat no : 12074-1-AP

Synonyms

CGI 104, HSPC172, PTD009, SBDN, SYNBINDIN, TRAPPC4, TRS23, TRS23 homolog

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using TRAPPC4 Polyclonal antibody (12074-1-AP)

    WB analysis using 12074-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 12074-1-AP (TRAPPC4 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of mouse testis tissue using TRAPPC4 Polyclonal antibody (12074-1-AP)

    WB analysis of mouse testis using 12074-1-AP

    mouse testis tissue were subjected to SDS PAGE followed by western blot with 12074-1-AP (TRAPPC4 antibody) at dilution of 1:1000 incubated at 4 degree celsius over night.

  • Immunoprecipitation (IP) experiment of HL-60 cells using TRAPPC4 Polyclonal antibody (12074-1-AP)

    IP experiment of HL-60 using 12074-1-AP

    IP result of anti-TRAPPC4 (IP:12074-1-AP, 4ug; Detection:12074-1-AP 1:800) with HL-60 cells lysate 1720 ug.

  • Immunohistochemistry (IHC) staining of human colon cancer tissue using TRAPPC4 Polyclonal antibody (12074-1-AP)

    IHC staining of human colon cancer using 12074-1-AP

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 12074-1-AP (TRAPPC4 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human colon cancer tissue using TRAPPC4 Polyclonal antibody (12074-1-AP)

    IHC staining of human colon cancer using 12074-1-AP

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 12074-1-AP (TRAPPC4 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using TRAPPC4 Polyclonal antibody (12074-1-AP)

    IF Staining of HeLa using 12074-1-AP

    Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using TRAPPC4 antibody (12074-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

Tested Applications

Positive WB detected inHL-60 cells, mouse testis tissue, rat testis tissue
Positive IP detected inHL-60 cells
Positive IHC detected inhuman colon cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:4000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:500-1:2000
Immunofluorescence (IF)IF : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

12074-1-AP targets TRAPPC4 in WB, IP, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen TRAPPC4 fusion protein Ag2737
Full Name trafficking protein particle complex 4
Calculated Molecular Weight 219 aa, 24 kDa
Observed Molecular Weight 24-27 kDa
GenBank Accession NumberBC010866
Gene Symbol TRAPPC4
Gene ID (NCBI) 51399
RRIDAB_2208147
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Transport protein particle (TRAPP, also known as trafficking protein particle), a multimeric guanine nucleotide-exchange factor, regulates multiple membrane trafficking pathways (PMID: 20966969). TRAPPC4, also known as synbindin, is a core component of the TRAPP complexes and one of the essential subunits for guanine nucleotide exchange factor activity for Rab1 GTPase (PMID: 31794024). Deficiencies in vesicular transport mediated by TRAPPC4 have been associated with severe syndromic intellectual disability (PMID: 31794024).

Protocols

Product Specific Protocols
WB protocol for TRAPPC4 antibody 12074-1-APDownload protocol
IHC protocol for TRAPPC4 antibody 12074-1-APDownload protocol
IF protocol for TRAPPC4 antibody 12074-1-APDownload protocol
IP protocol for TRAPPC4 antibody 12074-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols