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TRAT1 Polyclonal antibody

TRAT1 Polyclonal Antibody for WB, IP, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, IHC, ELISA

Conjugate

Unconjugated

Cat no : 13279-1-AP

Synonyms

HSPC062, pp29/30, TCRIM, TRAT1, TRIM

Validation Data Gallery

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  • Western Blot (WB) analysis of mouse thymus tissue using TRAT1 Polyclonal antibody (13279-1-AP)

    WB analysis of mouse thymus using 13279-1-AP

    mouse thymus tissue were subjected to SDS PAGE followed by western blot with 13279-1-AP (TRAT1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of Jurkat cells using TRAT1 Polyclonal antibody (13279-1-AP)

    WB analysis of Jurkat using 13279-1-AP

    Jurkat cells were subjected to SDS PAGE followed by western blot with 13279-1-AP (TRAT1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Immunoprecipitation (IP) experiment of Jurkat cells using TRAT1 Polyclonal antibody (13279-1-AP)

    IP experiment of Jurkat using 13279-1-AP

    IP result of anti-TRAT1 (IP:13279-1-AP, 4ug; Detection:13279-1-AP 1:300) with Jurkat cells lysate 3000 ug.

  • Immunohistochemistry (IHC) staining of human tonsillitis tissue using TRAT1 Polyclonal antibody (13279-1-AP)

    IHC staining of human tonsillitis using 13279-1-AP

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 13279-1-AP (TRAT1 Antibody) at dilution of 1:100 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human tonsillitis tissue using TRAT1 Polyclonal antibody (13279-1-AP)

    IHC staining of human tonsillitis using 13279-1-AP

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 13279-1-AP (TRAT1 Antibody) at dilution of 1:100 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

Tested Applications

Positive WB detected inmouse thymus tissue, Jurkat cells
Positive IP detected inJurkat cells
Positive IHC detected inhuman tonsillitis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:4000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

13279-1-AP targets TRAT1 in WB, IP, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen TRAT1 fusion protein Ag4023
Full Name T cell receptor associated transmembrane adaptor 1
Calculated Molecular Weight 186 aa, 21 kDa
Observed Molecular Weight 29 kDa
GenBank Accession NumberBC025713
Gene Symbol TRAT1
Gene ID (NCBI) 50852
RRIDAB_10643524
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Protocols

Product Specific Protocols
WB protocol for TRAT1 antibody 13279-1-APDownload protocol
IHC protocol for TRAT1 antibody 13279-1-APDownload protocol
IP protocol for TRAT1 antibody 13279-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols