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TRIM40 Monoclonal antibody

TRIM40 Monoclonal Antibody for IHC, WB,ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human, Pig and More (1)

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

CloneNo.

1C5D3

Cat no : 67073-1-Ig

Synonyms

RING finger protein 35, RNF35, TRIM40, tripartite motif containing 40

Validation Data Gallery

Filter:
  • Western Blot (WB) analysis of various lysates using TRIM40 Monoclonal antibody (67073-1-Ig)

    WB analysis using 67073-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 67073-1-Ig (TRIM40 antibody) at dilution of 1:1400 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of pig small intestine tissue using TRIM40 Monoclonal antibody (67073-1-Ig)

    WB analysis of pig small intestine using 67073-1-Ig

    pig small intestine tissue were subjected to SDS PAGE followed by western blot with 67073-1-Ig (TRIM40 antibody) at dilution of 1:1400 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of various lysates using TRIM40 Monoclonal antibody (67073-1-Ig)

    WB analysis using 67073-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 67073-1-Ig (TRIM40 antibody) at dilution of 1:1400 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human small intestine tissue using TRIM40 Monoclonal antibody (67073-1-Ig)

    IHC staining of human small intestine using 67073-1-Ig

    Immunohistochemical analysis of paraffin-embedded human small intestine tissue slide using 67073-1-Ig (TRIM40 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human small intestine tissue using TRIM40 Monoclonal antibody (67073-1-Ig)

    IHC staining of human small intestine using 67073-1-Ig

    Immunohistochemical analysis of paraffin-embedded human small intestine tissue slide using 67073-1-Ig (TRIM40 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

Tested Applications

Positive WB detected inNCCIT cell, THP-1 cells, pig small intestine tissue, HT-29 cells, NCCIT cells, JAR cells
Positive IHC detected inhuman small intestine tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:600-1:3000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

WBSee 1 publications below

Product Information

67073-1-Ig targets TRIM40 in WB, IHC, ELISA applications and shows reactivity with Human, Pig samples.

Tested Reactivity Human, Pig
Cited Reactivityhuman
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen TRIM40 fusion protein Ag21660
Full Name tripartite motif-containing 40
Calculated Molecular Weight 258 aa, 29 kDa
Observed Molecular Weight 26-36 kDa
GenBank Accession NumberBC060785
Gene Symbol TRIM40
Gene ID (NCBI) 135644
RRIDAB_2882382
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Protocols

Product Specific Protocols
WB protocol for TRIM40 antibody 67073-1-IgDownload protocol
IHC protocol for TRIM40 antibody 67073-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Cell Rep

Type I interferon/STAT1 signaling regulates UBE2M-mediated antiviral innate immunity in a negative feedback manner

Authors - Xianghui Kong
View Article