TRPM8 Polyclonal antibody
TRPM8 Polyclonal Antibody for IF, IHC,ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human and More (1)
Applications
IHC, IF, ELISA
Conjugate
Unconjugated
Cat no : 12813-1-AP
Synonyms
Validation Data Gallery
Tested Applications
| Positive IHC detected in | human liver cancer tissue, human prostate cancer tissue, human colon cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF detected in | SH-SY5Y cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| Immunofluorescence (IF) | IF : 1:10-1:100 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| IHC | See 2 publications below |
| IF | See 3 publications below |
Product Information
12813-1-AP targets TRPM8 in IHC, IF, ELISA applications and shows reactivity with human samples.
| Tested Reactivity | human |
| Cited Reactivity | human, mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | TRPM8 fusion protein Ag3483 |
| Full Name | transient receptor potential cation channel, subfamily M, member 8 |
| Calculated Molecular Weight | 1104 aa, 128 kDa |
| GenBank Accession Number | BC001135 |
| Gene Symbol | TRPM8 |
| Gene ID (NCBI) | 79054 |
| RRID | AB_2877883 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
TRPM8, also known as CMR1 (cold-menthol receptor-1), is a nonselective cation channel activated by cold and the cooling substances menthol and icilin (PMID: 11893340; 11882888). It serves as a neuronal sensor of cold temperatures. Functioning TRPM8 channels are required for behavioral responses to innocuous cool, noxious cold, injury-evoked cold hypersensitivity, and cooling-mediated analgesia (PMID: 17538622; 21438154; 16920620; 21984952). Additionally, TRPM8 has also been reported to be involved in thermoregulation and the regulation of basal tear flow (PMID: 21407809; 21076394). TRPM8 is expressed extensively in sensory nerves, human prostate and overexpressed in a variety of cancers including prostate, breast, lung, colon and skin melanomas (PMID: 11325849; 20816009).
Protocols
| Product Specific Protocols | |
|---|---|
| IHC protocol for TRPM8 antibody 12813-1-AP | Download protocol |
| IF protocol for TRPM8 antibody 12813-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Biomaterials Role of molybdenum in material immunomodulation and periodontal wound healing: Targeting immunometabolism and mitochondrial function for macrophage modulation. | ||
Inflammation Cold Stimuli Facilitate Inflammatory Responses Through Transient Receptor Potential Melastatin 8 (TRPM8) in Primary Airway Epithelial Cells of Asthmatic Mice. | ||
J Dermatol Sci The temperature-sensitive receptors TRPV4 and TRPM8 have important roles in the pruritus of rosacea | ||
Cancer Gene Ther TRPM8 promotes hepatocellular carcinoma progression by inducing SNORA55 mediated nuclear-mitochondrial communication |
