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TSN Polyclonal antibody

TSN Polyclonal Antibody for WB, IP, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IP, IHC, ELISA

Conjugate

Unconjugated

Cat no : 55426-1-AP

Synonyms

REHF 1, translin, TRSLN, TSN

Validation Data Gallery

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  • Western Blot (WB) analysis of Jurkat cells using TSN Polyclonal antibody (55426-1-AP)

    WB analysis of Jurkat using 55426-1-AP

    Jurkat cells were subjected to SDS PAGE followed by western blot with 55426-1-AP (TSN Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HeLa cells using TSN Polyclonal antibody (55426-1-AP)

    WB analysis of HeLa using 55426-1-AP

    HeLa cells were subjected to SDS PAGE followed by western blot with 55426-1-AP (TSN Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of MCF-7 cells using TSN Polyclonal antibody (55426-1-AP)

    WB analysis of MCF-7 using 55426-1-AP

    MCF-7 cells were subjected to SDS PAGE followed by western blot with 55426-1-AP (TSN Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • Immunoprecipitation (IP) experiment of MCF-7 cells using TSN Polyclonal antibody (55426-1-AP)

    IP experiment of MCF-7 using 55426-1-AP

    IP result of anti-TSN (IP:55426-1-AP, 3ug; Detection:55426-1-AP 1:300) with MCF-7 cells lysate 1600ug.

  • Immunohistochemistry (IHC) staining of mouse testis tissue using TSN Polyclonal antibody (55426-1-AP)

    IHC staining of mouse testis using 55426-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 55426-1-AP (TSN antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of mouse brain tissue using TSN Polyclonal antibody (55426-1-AP)

    IHC staining of mouse brain using 55426-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 55426-1-AP (TSN antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

Tested Applications

Positive WB detected inJurkat cells, HeLa cells, MCF-7 cells
Positive IP detected inMCF-7 cells
Positive IHC detected inmouse testis tissue, mouse brain tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

55426-1-AP targets TSN in WB, IP, IHC, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Peptide
Full Name translin
Calculated Molecular Weight 26 kDa
Observed Molecular Weight 26 kDa
GenBank Accession NumberNM_004622
Gene Symbol TSN
Gene ID (NCBI) 7247
RRIDAB_2881326
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

TSN, also named as Translin or Component 3 of promoter of RISC, is a 228 amino acid protein, which belongs to the translin family. TSN as a DNA-binding protein that specifically recognizes consensus sequences at the breakpoint junctions in chromosomal translations, mostly is involved in immunoglobulin T-cell receptor gene segments. TSN May act as an activator of RNA-induced silencing complex (RISC) by facilitating endonucleolytic cleavage of the siRNA passenger strand.

Protocols

Product Specific Protocols
WB protocol for TSN antibody 55426-1-APDownload protocol
IHC protocol for TSN antibody 55426-1-APDownload protocol
IP protocol for TSN antibody 55426-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols