• All
  • Primary Antibodies
  • Conjugated Antibodies for IF
  • Conjugated Antibodies for FC
  • Secondary Antibodies
  • Antibody Labeling KitsNew
  • ELISA Kits
  • IHC Kits
  • Magnetic Cell Separation Kits
  • Cytokines & Growth Factors
  • Neutralizing/activating Antibodies
  • Nanobody-based Reagents
  • Accessory Products and Kits
  • Fusion Proteins
×
×
Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0
  • Featured Product
  • KD/KO Validated

TSPAN5 Polyclonal antibody

TSPAN5 Polyclonal Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, ELISA

Conjugate

Unconjugated

Cat no : 12122-1-AP

Synonyms

NET 4, NET4, Tetraspan NET 4, tetraspanin 5, TM4SF9, TSPAN 5, TSPAN5

Validation Data Gallery

Filter:
  • Western Blot (WB) analysis of Jurkat cells using TSPAN5 Polyclonal antibody (12122-1-AP)

    WB analysis of Jurkat using 12122-1-AP

    Jurkat cells were subjected to SDS PAGE followed by western blot with 12122-1-AP (TSPAN5 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of Jurkat cells using TSPAN5 Polyclonal antibody (12122-1-AP)

    WB analysis of Jurkat using 12122-1-AP

    WB result of TSPAN5 antibody (12122-1-AP; 1:2000; incubated at room temperature for 1.5 hours) with sh-Control and sh-TSPAN5 transfected Jurkat cells.

Tested Applications

Positive WB detected inJurkat cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:10000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

WBSee 1 publications below

Product Information

12122-1-AP targets TSPAN5 in WB, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Cited Reactivityhuman
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen TSPAN5 fusion protein Ag2765
Full Name tetraspanin 5
Calculated Molecular Weight 268 aa, 30 kDa
Observed Molecular Weight 30-40 kDa, 90 kDa
GenBank Accession NumberBC009704
Gene Symbol TSPAN5
Gene ID (NCBI) 10098
RRIDAB_10638611
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

TSPAN5 is a membrane protein of the tetraspanin superfamily, which are characterized by the presence of four conserved transmembrane regions. Tetraspanins have been involved in diverse processes such as cell activation and proliferation, adhesion and motility, differentiation, and cancer. TSPAN5 belongs to the TSPANC8 subfamily, which also include TSPAN10, TSPAN14, TSPAN15, TSPAN17, and TSPAN33. It has been reported that TSPANC8 tetraspanins interact with ADAM10 and have a conserved function in the regulation of ADAM10 trafficking and activity, thereby positively regulating Notch receptor activation (PMID: 23091066; 23035126).

Protocols

Product Specific Protocols
WB protocol for TSPAN5 antibody 12122-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Nat Commun

Phenylalanine-tRNA aminoacylation is compromised by ALS/FTD-associated C9orf72 C4G2 repeat RNA

Authors - Mirjana Malnar Črnigoj
View Article