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TSTA3 Polyclonal antibody

TSTA3 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 15335-1-AP

Synonyms

FX, GDP L fucose synthase, P35B, Protein FX, SDR4E1, TSTA3

Validation Data Gallery

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  • Western Blot (WB) analysis of human colon tissue using TSTA3 Polyclonal antibody (15335-1-AP)

    WB analysis of human colon using 15335-1-AP

    human colon tissue were subjected to SDS PAGE followed by western blot with 15335-1-AP (TSTA3 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of human brain tissue using TSTA3 Polyclonal antibody (15335-1-AP)

    WB analysis of human brain using 15335-1-AP

    human brain tissue were subjected to SDS PAGE followed by western blot with 15335-1-AP (TSTA3 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of human kidney tissue using TSTA3 Polyclonal antibody (15335-1-AP)

    WB analysis of human kidney using 15335-1-AP

    human kidney tissue were subjected to SDS PAGE followed by western blot with 15335-1-AP (TSTA3 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HeLa cells using TSTA3 Polyclonal antibody (15335-1-AP)

    WB analysis of HeLa using 15335-1-AP

    HeLa cells were subjected to SDS PAGE followed by western blot with 15335-1-AP (TSTA3 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HEK-293 cells using TSTA3 Polyclonal antibody (15335-1-AP)

    WB analysis of HEK-293 using 15335-1-AP

    HEK-293 cells were subjected to SDS PAGE followed by western blot with 15335-1-AP (TSTA3 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human kidney tissue using TSTA3 Polyclonal antibody (15335-1-AP)

    IHC staining of human kidney using 15335-1-AP

    Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 15335-1-AP (TSTA3 Antibody) at dilution of 1:200 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human kidney tissue using TSTA3 Polyclonal antibody (15335-1-AP)

    IHC staining of human kidney using 15335-1-AP

    Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 15335-1-AP (TSTA3 Antibody) at dilution of 1:200 (under 40x lens).

Tested Applications

Positive WB detected inhuman colon tissue, HEK-293 cells, HeLa cells, human brain tissue, human kidney tissue
Positive IHC detected inhuman kidney tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:100-1:400
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

15335-1-AP targets TSTA3 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen TSTA3 fusion protein Ag7450
Full Name tissue specific transplantation antigen P35B
Calculated Molecular Weight 36 kDa
Observed Molecular Weight 40 kDa, 68 kDa
GenBank Accession NumberBC001941
Gene Symbol TSTA3
Gene ID (NCBI) 7264
RRIDAB_2211226
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Protocols

Product Specific Protocols
WB protocol for TSTA3 antibody 15335-1-APDownload protocol
IHC protocol for TSTA3 antibody 15335-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols