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TUFM Polyclonal antibody

TUFM Polyclonal Antibody for FC, IF, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (1)

Applications

WB, IF, FC, ELISA

Conjugate

Unconjugated

Cat no : 26730-1-AP

Synonyms

COXPD4, EF Tu, EF TuMT, EFTU, P43, TUFM

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using TUFM Polyclonal antibody (26730-1-AP)

    WB analysis using 26730-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 26730-1-AP (TUFM antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using TUFM Polyclonal antibody (26730-1-AP)

    IF Staining of HepG2 using 26730-1-AP

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using 26730-1-AP (TUFM antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using TUFM Polyclonal antibody (26730-1-AP)

    IF Staining of HepG2 using 26730-1-AP

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using TUFM antibody (26730-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using TUFM Polyclonal antibody (26730-1-AP)

    IF Staining of HepG2 using 26730-1-AP

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using TUFM antibody (26730-1-AP) at dilution of 1:1000 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-phalloidin (red).

  • Flow cytometry (FC) experiment of HepG2 cells using TUFM Polyclonal antibody (26730-1-AP)

    FC experiment of HepG2 using 26730-1-AP

    1X10^6 HepG2 cells were intracellularly stained with 0.4 ug Anti-Human TUFM (26730-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Isotype Control. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

Tested Applications

Positive WB detected inA431 cells, Jurkat cells, mouse brain tissue, rat brain tissue
Positive IF detected inHepG2 cells
Positive FC detected inHepG2 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:8000
Immunofluorescence (IF)IF : 1:50-1:500
Flow Cytometry (FC)FC : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

WBSee 4 publications below

Product Information

26730-1-AP targets TUFM in WB, IF, FC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityrat, zebrafish
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen TUFM fusion protein Ag24981
Full Name Tu translation elongation factor, mitochondrial
Calculated Molecular Weight 50 kDa
Observed Molecular Weight 46-50 kDa
GenBank Accession NumberBC010041
Gene Symbol TUFM
Gene ID (NCBI) 7284
RRIDAB_2880616
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

TUFM, also named as Elongation factor Tu, mitochondrial, is a 452 amino acid protein, which contains 1 tr-type G (guanine nucleotide-binding) domain and belongs to the TRAFAC class translation factor GTPase superfamily. TUFM localizes in the Mitochondrion and promotes the GTP-dependent binding of aminoacyl-tRNA to the A-site of ribosomes during protein biosynthesis.

Protocols

Product Specific Protocols
WB protocol for TUFM antibody 26730-1-APDownload protocol
IF protocol for TUFM antibody 26730-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
zebrafishWB

Nucleic Acids Res

Deletion of Mtu1 (Trmu) in zebrafish revealed the essential role of tRNA modification in mitochondrial biogenesis and hearing function.

Authors - Qinghai Zhang
View Article
zebrafishWB

Nucleic Acids Res

Ablation of Mto1 in zebrafish exhibited hypertrophic cardiomyopathy manifested by mitochondrion RNA maturation deficiency.

Authors - Qinghai Zhang
View Article
ratWB

Sci Total Environ

Fluoride impairs mitochondrial translation by targeting miR-221-3p/c-Fos/RMND1 axis contributing to neurodevelopment defects

Authors - Dongjie Li
View Article
zebrafishWB

Nucleic Acids Res

Deletion of Gtpbp3 in zebrafish revealed the hypertrophic cardiomyopathy manifested by aberrant mitochondrial tRNA metabolism.

Authors - Danni Chen
View Article