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TXNDC3 Polyclonal antibody

TXNDC3 Polyclonal Antibody for IP, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, ELISA

Conjugate

Unconjugated

Cat no : 13586-1-AP

Synonyms

CILD6, NME8, Sptrx 2, SPTRX2, TXNDC3

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using TXNDC3 Polyclonal antibody (13586-1-AP)

    WB analysis using 13586-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 13586-1-AP (TXNDC3 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of human testis tissue using TXNDC3 Polyclonal antibody (13586-1-AP)

    WB analysis of human testis using 13586-1-AP

    human testis tissue were subjected to SDS PAGE followed by western blot with 13586-1-AP (TXNDC3 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of mouse testis tissue using TXNDC3 Polyclonal antibody (13586-1-AP)

    WB analysis of mouse testis using 13586-1-AP

    mouse testis tissue were subjected to SDS PAGE followed by western blot with 13586-1-AP (TXNDC3 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Immunoprecipitation (IP) experiment of mouse testis tissue using TXNDC3 Polyclonal antibody (13586-1-AP)

    IP experiment of mouse testis using 13586-1-AP

    IP result of anti-TXNDC3 (IP:13586-1-AP, 4ug; Detection:13586-1-AP 1:1000) with mouse testis tissue lysate 4000ug.

Tested Applications

Positive WB detected inmouse testis tissue, human testis tissue, rat testis tissue
Positive IP detected inmouse testis tissue

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:3000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

13586-1-AP targets TXNDC3 in WB, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen TXNDC3 fusion protein Ag4514
Full Name thioredoxin domain containing 3 (spermatozoa)
Calculated Molecular Weight 588 aa, 67 kDa
Observed Molecular Weight 67 kDa
GenBank Accession NumberBC036816
Gene Symbol TXNDC3
Gene ID (NCBI) 51314
RRIDAB_2212304
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Protocols

Product Specific Protocols
WB protocol for TXNDC3 antibody 13586-1-APDownload protocol
IP protocol for TXNDC3 antibody 13586-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols