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UACA Polyclonal antibody

UACA Polyclonal Antibody for IF, IHC, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse and More (1)

Applications

WB, IHC, IF, ELISA

Conjugate

Unconjugated

Cat no : 25654-1-AP

Synonyms

KIAA1561, NUCLING, UACA

Validation Data Gallery

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  • Western Blot (WB) analysis of HeLa cells using UACA Polyclonal antibody (25654-1-AP)

    WB analysis of HeLa using 25654-1-AP

    HeLa cells were subjected to SDS PAGE followed by western blot with 25654-1-AP (UACA Antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of A375 cells using UACA Polyclonal antibody (25654-1-AP)

    WB analysis of A375 using 25654-1-AP

    A375 cells were subjected to SDS PAGE followed by western blot with 25654-1-AP (UACA Antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of Y79 cells using UACA Polyclonal antibody (25654-1-AP)

    WB analysis of Y79 using 25654-1-AP

    Y79 cells were subjected to SDS PAGE followed by western blot with 25654-1-AP (UACA Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of Neuro-2a cells using UACA Polyclonal antibody (25654-1-AP)

    WB analysis of Neuro-2a using 25654-1-AP

    Neuro-2a cells were subjected to SDS PAGE followed by western blot with 25654-1-AP (UACA Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HEK-293 cells using UACA Polyclonal antibody (25654-1-AP)

    WB analysis of HEK-293 using 25654-1-AP

    HEK-293 cells were subjected to SDS PAGE followed by western blot with 25654-1-AP (UACA Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of Neuro-2a cells using UACA Polyclonal antibody (25654-1-AP)

    WB analysis of Neuro-2a using 25654-1-AP

    Neuro-2a cells were subjected to SDS PAGE followed by western blot with 25654-1-AP (UACA Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of Y79 cells using UACA Polyclonal antibody (25654-1-AP)

    WB analysis of Y79 using 25654-1-AP

    Y79 cells were subjected to SDS PAGE followed by western blot with 25654-1-AP (UACA Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human colon tissue using UACA Polyclonal antibody (25654-1-AP)

    IHC staining of human colon using 25654-1-AP

    Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 25654-1-AP (UACA antibody) at dilution of 1:200 (under 40x lens).

  • Immunohistochemistry (IHC) staining of human colon tissue using UACA Polyclonal antibody (25654-1-AP)

    IHC staining of human colon using 25654-1-AP

    Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 25654-1-AP (UACA antibody) at dilution of 1:200 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human kidney tissue using UACA Polyclonal antibody (25654-1-AP)

    IHC staining of human kidney using 25654-1-AP

    Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 25654-1-AP (UACA antibody) at dilution of 1:200 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human kidney tissue using UACA Polyclonal antibody (25654-1-AP)

    IHC staining of human kidney using 25654-1-AP

    Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 25654-1-AP (UACA antibody) at dilution of 1:200 (under 40x lens).

  • Immunofluorescence (IF) / fluorescent staining of A375 cells using UACA Polyclonal antibody (25654-1-AP)

    IF Staining of A375 using 25654-1-AP

    Immunofluorescent analysis of A375 cells using 25654-1-AP (UACA antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

Tested Applications

Positive WB detected inHeLa cells, A375 cells, HEK-293 cells, Neuro-2a cells, Y79 cells
Positive IHC detected inhuman colon tissue, human kidney tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inA375 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:4000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)IF : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

WBSee 3 publications below

Product Information

The immunogen of 25654-1-AP is UACA Fusion Protein expressed in E. coli.

Tested Reactivity human, mouse
Cited Reactivityhuman, canine
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen UACA fusion protein Ag22438
Full Name uveal autoantigen with coiled-coil domains and ankyrin repeats
Calculated Molecular Weight 1416 aa, 163 kDa
Observed Molecular Weight 163 kDa
GenBank Accession NumberBC113407
Gene Symbol UACA
Gene ID (NCBI) 55075
RRIDAB_2880179
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Protocols

Product Specific Protocols
WB protocol for UACA antibody 25654-1-APDownload protocol
IHC protocol for UACA antibody 25654-1-APDownload protocol
IF protocol for UACA antibody 25654-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Aging (Albany NY)

Transcriptome and proteome analysis of the antitumor activity of maslinic acid against pancreatic cancer cells

Authors - Hewei Zhang
View Article
humanWB

J Oncol

Maslinic Acid Inhibits the Growth of Malignant Gliomas by Inducing Apoptosis via MAPK Signaling.

Authors - Yongqiang Wang
View Article
canineWB

Cell Rep

Rab39 and its effector UACA regulate basolateral exosome release from polarized epithelial cells.

Authors - Takahide Matsui
View Article