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UAP1L1 Polyclonal antibody

UAP1L1 Polyclonal Antibody for WB, IF, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IF, ELISA

Conjugate

Unconjugated

Cat no : 25262-1-AP

Synonyms

UAP1L1

Validation Data Gallery

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  • Western Blot (WB) analysis of MDA-MB-453s cells using UAP1L1 Polyclonal antibody (25262-1-AP)

    WB analysis of MDA-MB-453s using 25262-1-AP

    MDA-MB-453s cells were subjected to SDS PAGE followed by western blot with 25262-1-AP (UAP1L1 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HepG2 cells using UAP1L1 Polyclonal antibody (25262-1-AP)

    WB analysis of HepG2 using 25262-1-AP

    HepG2 cells were subjected to SDS PAGE followed by western blot with 25262-1-AP (UAP1L1 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of A375 cells using UAP1L1 Polyclonal antibody (25262-1-AP)

    WB analysis of A375 using 25262-1-AP

    A375 cells were subjected to SDS PAGE followed by western blot with 25262-1-AP (UAP1L1 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of K-562 cells using UAP1L1 Polyclonal antibody (25262-1-AP)

    WB analysis of K-562 using 25262-1-AP

    K-562 cells were subjected to SDS PAGE followed by western blot with 25262-1-AP (UAP1L1 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using UAP1L1 Polyclonal antibody (25262-1-AP)

    IF Staining of HepG2 using 25262-1-AP

    Immunofluorescent analysis of HepG2 cells using 25262-1-AP (UAP1L1 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

Tested Applications

Positive WB detected inMDA-MB-453s cells, A375 cells, HepG2 cells, K-562 cells
Positive IF detected inHepG2 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunofluorescence (IF)IF : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

25262-1-AP targets UAP1L1 in WB, IF, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Peptide
Full Name UDP-N-acteylglucosamine pyrophosphorylase 1-like 1
Calculated Molecular Weight 57 kDa
Observed Molecular Weight 57-59 kDa
GenBank Accession NumberNM_207309
Gene Symbol UAP1L1
Gene ID (NCBI) 91373
RRIDAB_2879993
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Protocols

Product Specific Protocols
WB protocol for UAP1L1 antibody 25262-1-APDownload protocol
IF protocol for UAP1L1 antibody 25262-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols