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UBAC1 Polyclonal antibody

UBAC1 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 19844-1-AP

Synonyms

GBDR1, KPC2, RP11 432J22.3, UBA domain containing 1, UBAC1, UBADC1

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using UBAC1 Polyclonal antibody (19844-1-AP)

    WB analysis using 19844-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 19844-1-AP (UBAC1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of mouse brain tissue using UBAC1 Polyclonal antibody (19844-1-AP)

    WB analysis of mouse brain using 19844-1-AP

    mouse brain tissue were subjected to SDS PAGE followed by western blot with 19844-1-AP (UBAC1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of rat liver tissue using UBAC1 Polyclonal antibody (19844-1-AP)

    WB analysis of rat liver using 19844-1-AP

    rat liver tissue were subjected to SDS PAGE followed by western blot with 19844-1-AP (UBAC1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of mouse heart tissue using UBAC1 Polyclonal antibody (19844-1-AP)

    WB analysis of mouse heart using 19844-1-AP

    mouse heart tissue were subjected to SDS PAGE followed by western blot with 19844-1-AP (UBAC1 Antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human heart tissue using UBAC1 Polyclonal antibody (19844-1-AP)

    IHC staining of human heart using 19844-1-AP

    Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 19844-1-AP (UBAC1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human heart tissue using UBAC1 Polyclonal antibody (19844-1-AP)

    IHC staining of human heart using 19844-1-AP

    Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 19844-1-AP (UBAC1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human heart tissue using UBAC1 Polyclonal antibody (19844-1-AP)

    IHC staining of human heart using 19844-1-AP

    Immunohistochemical analysis of paraffin-embedded human heart using 19844-1-AP (UBAC1 antibody) at dilution of 1:100 (under 40x lens).

Tested Applications

Positive WB detected inmouse brain tissue, rat liver tissue, mouse heart tissue, rat brain tissue, rat heart tissue
Positive IHC detected inhuman heart tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:4000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

The immunogen of 19844-1-AP is UBAC1 Fusion Protein expressed in E. coli.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen UBAC1 fusion protein Ag13887
Full Name UBA domain containing 1
Calculated Molecular Weight 405 aa, 45 kDa
Observed Molecular Weight 46 kDa
GenBank Accession NumberBC004967
Gene Symbol UBAC1
Gene ID (NCBI) 10422
RRIDAB_10638923
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

UBAC1(Ubiquitin-associated domain-containing protein 1) is also named as GBDR1, KPC2, UBADC1. It has several putative phosphorylation sites, including 8 CK2 (see 115440) sites, as well as 1 N-glycosylation site and 2 N-myristoylation sites(PMID:10857748). It is required for poly-ubiquitination and proteasome-mediated degradation of CDKN1B during G1 phase of the cell cycle.Northern blot analysis shows UBAC1 expression with the highest levels in heart and skeletal muscle and lowest level in lung. This antibody is specific to UBAC1.

Protocols

Product Specific Protocols
WB protocol for UBAC1 antibody 19844-1-APDownload protocol
IHC protocol for UBAC1 antibody 19844-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols