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  • Featured Product
  • KD/KO Validated

UBAC2 Polyclonal antibody

UBAC2 Polyclonal Antibody for WB, IF, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IF, RIP, IHC, ELISA

Conjugate

Unconjugated

Cat no : 25122-1-AP

Synonyms

PHGDHL1, UBA domain containing 2, UBAC2

Validation Data Gallery

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  • Western Blot (WB) analysis of MCF-7 cells using UBAC2 Polyclonal antibody (25122-1-AP)

    WB analysis of MCF-7 using 25122-1-AP

    MCF-7 cells were subjected to SDS PAGE followed by western blot with 25122-1-AP (UBAC2 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HEK-293 cells using UBAC2 Polyclonal antibody (25122-1-AP)

    WB analysis of HEK-293 using 25122-1-AP

    HEK-293 cells were subjected to SDS PAGE followed by western blot with 25122-1-AP (UBAC2 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of mouse kidney tissue using UBAC2 Polyclonal antibody (25122-1-AP)

    WB analysis of mouse kidney using 25122-1-AP

    mouse kidney tissue were subjected to SDS PAGE followed by western blot with 25122-1-AP (UBAC2 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human colon cancer tissue using UBAC2 Polyclonal antibody (25122-1-AP)

    IHC staining of human colon cancer using 25122-1-AP

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 25122-1-AP (UBAC2 antibody) at dilution of 1:200 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human colon cancer tissue using UBAC2 Polyclonal antibody (25122-1-AP)

    IHC staining of human colon cancer using 25122-1-AP

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 25122-1-AP (UBAC2 antibody) at dilution of 1:200 (under 40x lens).

  • Immunohistochemistry (IHC) staining of human testis tissue using UBAC2 Polyclonal antibody (25122-1-AP)

    IHC staining of human testis using 25122-1-AP

    Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 25122-1-AP (UBAC2 antibody) at dilution of 1:200 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human testis tissue using UBAC2 Polyclonal antibody (25122-1-AP)

    IHC staining of human testis using 25122-1-AP

    Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 25122-1-AP (UBAC2 antibody) at dilution of 1:200 (under 40x lens).

  • Immunofluorescence (IF) / fluorescent staining of HEK-293 cells using UBAC2 Polyclonal antibody (25122-1-AP)

    IF Staining of HEK-293 using 25122-1-AP

    Immunofluorescent analysis of HEK-293 cells using 25122-1-AP (UBAC2 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

Tested Applications

Positive WB detected inMCF-7 cells, HEK-293 cells, mouse kidney tissue
Positive IHC detected inhuman colon cancer tissue, human testis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHEK-293 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)IF : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

The immunogen of 25122-1-AP is UBAC2 Fusion Protein expressed in E. coli.

Tested Reactivity human, mouse
Cited Reactivityhuman
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen UBAC2 fusion protein Ag19142
Full Name UBA domain containing 2
Calculated Molecular Weight 344 aa, 39 kDa
Observed Molecular Weight 35 kDa
GenBank Accession NumberBC121139
Gene Symbol UBAC2
Gene ID (NCBI) 337867
RRIDAB_2879910
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Protocols

Product Specific Protocols
WB protocol for UBAC2 antibody 25122-1-APDownload protocol
IHC protocol for UBAC2 antibody 25122-1-APDownload protocol
IF protocol for UBAC2 antibody 25122-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

J Extracell Vesicles

A multi-omics approach identifies pancreatic cancer cell extracellular vesicles as mediators of the unfolded protein response in normal pancreatic epithelial cells.

Authors - Charles P Hinzman
View Article
humanWB,IHC,IF,RIP

Cell Death Dis

UBAC2 promotes bladder cancer proliferation through BCRC-3/miRNA-182-5p/p27 axis.

Authors - Chaohui Gu
  • KD Validated
View Article
humanWB

Mol Cell

TMUB1 is an endoplasmic reticulum-resident escortase that promotes the p97-mediated extraction of membrane proteins for degradation

Authors - Linhan Wang
View Article