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UBL4B Polyclonal antibody

UBL4B Polyclonal Antibody for WB, IF, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IF, ELISA

Conjugate

Unconjugated

Cat no : 14509-1-AP

Synonyms

ubiquitin like 4B, Ubiquitin like protein 4B, UBL4B

Validation Data Gallery

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  • Western Blot (WB) analysis of human testis tissue using UBL4B Polyclonal antibody (14509-1-AP)

    WB analysis of human testis using 14509-1-AP

    human testis tissue were subjected to SDS PAGE followed by western blot with 14509-1-AP (UBL4B antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of human testis tissue using UBL4B Polyclonal antibody (14509-1-AP)

    WB analysis of human testis using 14509-1-AP

    human testis tissue were subjected to SDS PAGE followed by western blot with 14509-1-AP (UBL4B antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using UBL4B Polyclonal antibody (14509-1-AP)

    IF Staining of HepG2 using 14509-1-AP

    Immunofluorescent analysis of HepG2 cells, using UBL4B antibody 14509-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red). Blue pseudocolor = DAPI (fluorescent DNA dye).

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using UBL4B Polyclonal antibody (14509-1-AP)

    IF Staining of HeLa using 14509-1-AP

    Immunofluorescent analysis of Hela cells, using UBL4B antibody 14509-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red). Blue pseudocolor = DAPI (fluorescent DNA dye).

Tested Applications

Positive WB detected inhuman testis tissue
Positive IF detected inHepG2 cells, Hela cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunofluorescence (IF)IF : 1:10-1:100
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

WBSee 1 publications below

Product Information

14509-1-AP targets UBL4B in WB, IF, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen UBL4B fusion protein Ag5968
Full Name ubiquitin-like 4B
Calculated Molecular Weight 20 kDa
Observed Molecular Weight 22-25 kDa
GenBank Accession NumberBC058929
Gene Symbol UBL4B
Gene ID (NCBI) 164153
RRIDAB_10640905
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Protocols

Product Specific Protocols
WB protocol for UBL4B antibody 14509-1-APDownload protocol
IF protocol for UBL4B antibody 14509-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Andrology

The MAEL expression in mitochondria of human spermatozoa and the association with asthenozoospermia

Authors - Yu-Sheng Cheng
View Article