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UBTD2 Polyclonal antibody

UBTD2 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 13172-1-AP

Synonyms

DCUBP, DC-UbP, ubiquitin domain containing 2, Ubiquitin like protein SB72, UBTD2

Validation Data Gallery

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  • Western Blot (WB) analysis of human liver tissue using UBTD2 Polyclonal antibody (13172-1-AP)

    WB analysis of human liver using 13172-1-AP

    human liver tissue were subjected to SDS PAGE followed by western blot with 13172-1-AP (UBTD2 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human breast cancer tissue using UBTD2 Polyclonal antibody (13172-1-AP)

    IHC staining of human breast cancer using 13172-1-AP

    Immunohistochemical analysis of paraffin-embedded human breast cancer using 13172-1-AP (UBTD2 antibody) at dilution of 1:100 (under 10x lens).

Tested Applications

Positive WB detected inhuman liver tissue
Positive IHC detected inhuman breast cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

WBSee 1 publications below

Product Information

13172-1-AP targets UBTD2 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityrat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen UBTD2 fusion protein Ag3827
Full Name ubiquitin domain containing 2
Calculated Molecular Weight 234 aa, 26 kDa
Observed Molecular Weight 26 kDa
GenBank Accession NumberBC019910
Gene Symbol UBTD2
Gene ID (NCBI) 92181
RRIDAB_2210317
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Dendritic cell-derived ubiquitin (Ub)-like protein (DC-UbP), also named as Ub domain-containing protein 2 (UBTD2), is a potential Ub shuttle protein comprised of a Ub-like (UbL) domain and a Ub-binding domain (UBD), but its biological function remains largely unknown.PMID: 25207809

Protocols

Product Specific Protocols
WB protocol for UBTD2 antibody 13172-1-APDownload protocol
IHC protocol for UBTD2 antibody 13172-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
ratWB

Ann N Y Acad Sci

miR-141-3p affects β-catenin signaling and apoptosis by targeting Ubtd2 in rats with anorectal malformations

Authors - Chen Yi Wang
View Article