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UBXN11 Polyclonal antibody

UBXN11 Polyclonal Antibody for WB, IP, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, IHC, ELISA

Conjugate

Unconjugated

Cat no : 13109-1-AP

Synonyms

COA 1, PP2243, SOC, SOCI, Socius, UBX domain protein 11, UBXD5, UBXN11

Validation Data Gallery

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  • Western Blot (WB) analysis of PC-3 cells using UBXN11 Polyclonal antibody (13109-1-AP)

    WB analysis of PC-3 using 13109-1-AP

    PC-3 cells were subjected to SDS PAGE followed by western blot with 13109-1-AP (UBXN11 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of mouse testis tissue using UBXN11 Polyclonal antibody (13109-1-AP)

    WB analysis of mouse testis using 13109-1-AP

    mouse testis tissue were subjected to SDS PAGE followed by western blot with 13109-1-AP (UBXN11 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Immunoprecipitation (IP) experiment of mouse testis tissue using UBXN11 Polyclonal antibody (13109-1-AP)

    IP experiment of mouse testis using 13109-1-AP

    IP result of anti-UBXN11 (IP:13109-1-AP, 4ug; Detection:13109-1-AP 1:500) with mouse testis tissue lysate 4000ug.

  • Immunohistochemistry (IHC) staining of human testis tissue using UBXN11 Polyclonal antibody (13109-1-AP)

    IHC staining of human testis using 13109-1-AP

    Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 13109-1-AP (UBXN11 Antibody) at dilution of 1:200 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human testis tissue using UBXN11 Polyclonal antibody (13109-1-AP)

    IHC staining of human testis using 13109-1-AP

    Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 13109-1-AP (UBXN11 Antibody) at dilution of 1:200 (under 40x lens).

  • Immunohistochemistry (IHC) staining of human lung cancer tissue using UBXN11 Polyclonal antibody (13109-1-AP)

    IHC staining of human lung cancer using 13109-1-AP

    Immunohistochemical analysis of paraffin-embedded human lung cancer using 13109-1-AP (UBXN11 antibody) at dilution of 1:50 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human lung cancer tissue using UBXN11 Polyclonal antibody (13109-1-AP)

    IHC staining of human lung cancer using 13109-1-AP

    Immunohistochemical analysis of paraffin-embedded human lung cancer using 13109-1-AP (UBXN11 antibody) at dilution of 1:50 (under 40x lens).

  • Immunohistochemistry (IHC) staining of human testis tissue using UBXN11 Polyclonal antibody (13109-1-AP)

    IHC staining of human testis using 13109-1-AP

    Immunohistochemical analysis of paraffin-embedded human testis using 13109-1-AP (UBXN11 antibody) at dilution of 1:50 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human testis tissue using UBXN11 Polyclonal antibody (13109-1-AP)

    IHC staining of human testis using 13109-1-AP

    Immunohistochemical analysis of paraffin-embedded human testis using 13109-1-AP (UBXN11 antibody) at dilution of 1:50 (under 40x lens).

  • Immunohistochemistry (IHC) staining of human lung cancer tissue using UBXN11 Polyclonal antibody (13109-1-AP)

    IHC staining of human lung cancer using 13109-1-AP

    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 13109-1-AP (UBXN11 Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human lung cancer tissue using UBXN11 Polyclonal antibody (13109-1-AP)

    IHC staining of human lung cancer using 13109-1-AP

    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 13109-1-AP (UBXN11 Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

Tested Applications

Positive WB detected inPC-3 cells, mouse testis tissue
Positive IP detected inmouse testis tissue
Positive IHC detected inhuman testis tissue, human lung cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

13109-1-AP targets UBXN11 in WB, IP, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen UBXN11 fusion protein Ag3772
Full Name UBX domain protein 11
Calculated Molecular Weight 57 kDa
Observed Molecular Weight 48-54 kDa
GenBank Accession NumberBC038106
Gene Symbol UBXN11
Gene ID (NCBI) 91544
RRIDAB_2288037
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Protocols

Product Specific Protocols
WB protocol for UBXN11 antibody 13109-1-APDownload protocol
IHC protocol for UBXN11 antibody 13109-1-APDownload protocol
IP protocol for UBXN11 antibody 13109-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle

J Cell Sci

The p97-UBXN1 complex regulates aggresome formation.

Authors - Sirisha Mukkavalli
View Article