UNC119 Polyclonal antibody
UNC119 Polyclonal Antibody for IF, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
Canine and More (1)
Applications
IHC, IF, ELISA
Conjugate
Unconjugated
Cat no : 13065-1-AP
Synonyms
Validation Data Gallery
Tested Applications
| Positive IF detected in | MDCK cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Immunofluorescence (IF) | IF : 1:10-1:100 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| IHC | See 1 publications below |
Product Information
13065-1-AP targets UNC119 in IHC, IF, ELISA applications and shows reactivity with Canine samples.
| Tested Reactivity | Canine |
| Cited Reactivity | human |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | UNC119 fusion protein Ag3757 |
| Full Name | unc-119 homolog (C. elegans) |
| Calculated Molecular Weight | 240 aa, 27 kDa |
| GenBank Accession Number | BC027176 |
| Gene Symbol | UNC119 |
| Gene ID (NCBI) | 9094 |
| RRID | AB_2877911 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
Human UNC119 protein shares strong homology with the C. elegans unc119 protein which was first discovered in C. elegans on the basis of a spontaneous mutation affecting locomotion, feeding behavior and chemosensation. UNC119 is predominantly expressed in retina, in photoreceptor synapses and inner segments, with much lower expression in several other tissues. UNC119 is involved in the mechanism of photoreceptor neurotransmitter release through the synaptic vesicle cycle. It is required for G protein trafficking in sensory neurons.
Protocols
| Product Specific Protocols | |
|---|---|
| IF protocol for UNC119 antibody 13065-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Reviews
The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.
FH Francesc R (Verified Customer) (06-14-2022) | Antibody works nicely by Western blot, specifically recognizing band of roughly the expected size. Attached image shows blot of CRISPR clones: four still contain UNC119 and all the others without the band are the UNC119-KO clones.
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